FUT4 ELISA Kit (Human) (OKCD00264)

Catalog No: OKCD00264 (Formerly GWB-KBBGJ6)

Size:96WELLS

Human FUT4 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cluster Of Differentiation 15 (CD15). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cluster Of Differentiation 15 (CD15). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cluster Of Differentiation 15 (CD15), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Cluster Of Differentiation 15 (CD15) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Cluster Of Differentiation 15 (CD15) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Cluster Of Differentiation 15 (CD15) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-FUT4 Microplate: 96 Wells (12 x 8 Well Strips)
  • FUT4 Lyophilized Standard: 2 x 10 ng
  • 100X Biotinylated FUT4 Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Tissue homogenates, cell lysates and other biological fluids
• Sensitivity: < 0.058 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Fucosyltransferase 4 (FUT4). No significant cross-reactivity or interference between Fucosyltransferase 4 (FUT4) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: FUT4
• Gene Full Name: fucosyltransferase 4
• Alias Symbols: alpha (1,3) fucosyltransferase, myeloid-specific;alpha-(1,3)-fucosyltransferase 4;CD15;ELAM ligand fucosyltransferase;ELAM-1 ligand fucosyltransferase;ELFT;FCT3A;Fucosyltransferase 4;fucosyltransferase IV;fucT-IV;FUC-TIV;FUTIV;galactoside 3-L-fucosyltransferase;Lewis X;LeX;SSEA-1;stage-specific embryonic antigen 1.
• NCBI Gene Id: 2526
• Protein Name: Alpha-(1,3)-fucosyltransferase 4
• Description of Target: May catalyze alpha-1,3 glycosidic linkages involved in the expression of Lewis X/SSEA-1 and VIM-2 antigens.
• Uniprot ID: P22083
• Protein Accession #: NP_002024
• Nucleotide Accession #: NM_002033.3
• Protein Size (# AA): 530