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Catalog No: OKBB00459
Size:96 Wells
Price: $583.00
SKU
OKBB00459
Availability: Domestic: within 1-2 week delivery | International: within 1-2 week delivery
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHuman
ApplicationELISA
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration2.5 Hours
ELISA Kit PrincipleAviva's human Furin ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Furin has been precoated onto 96-well plates. Standards(NS0, D108-E715) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Furin is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the human Furin amount of sample captured in plate.
ELISA Kit Range312 pg/ml - 20,000 pg/ml
ELISA Kit Reproducibility
Intra-Assay PrecisionInter-Assay Precision
Sample123123
n 161616242424
Mean (pg/mL)1.98.815.72.39.217.4
SD0.0910.4930.9420.1220.5891.27
CV%4.85.665.36.47.3
ELISA Kit Component
ComponentAmount
96-Well plate Pre-coated with Anti-Human Furin Antibody1 x (12 x 8 Well Strip)
Lyophilized Recombinant Human Furin Standard2 x 20 ng/tube
100X Biotinylated Anti-Human Furin Antibody1 x 130 uL
100X Avidin-Biotin-Peroxidase Complex (ABC)1 x 130 uL
Sample Diluent Buffer1 x 30 mL
Antibody Diluent Buffer1 x 12 mL
ABC Diluent Buffer1 x 12 mL
TMB Color Developing Agent1 x 10 mL
TMB Stop Solution1 x 10 mL
10X Wash Buffer1 x 30 mL
Reconstitution and StorageStore at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Sample TypeCell culture supernatants, tissue homogenates and Cell lysates
Sensitivity<10 pg/ml
SpecificityNatural and recombinant human Furin
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
ImmunogenExpression system for standard: NS0; Immunogen sequence: D108-E715
DescriptionFor quantitative detection of Human Furin in cell culture supernatants and cell lysates.
Gene SymbolFURIN
Gene Full Namefurin (paired basic amino acid cleaving enzyme)
Alias SymbolsDibasic processing enzyme, Dibasic-processing enzyme, FES upstream region, FUR, FURIN, Furin membrane associated receptor protein, FURIN_HUMAN, PACE, Paired basic amino acid residue cleaving enzyme, Paired basic amino acid residue-cleaving enzyme, PCSK3, Proprotein convertase subtilisin/kexin type 3, SPC1
NCBI Gene Id5045
Protein NameFurin
Description of TargetFurin (Fur), also known as PACE, is a protein that in humans is encoded by the FURIN gene. The protein encoded by this gene is an enzyme which belongs to the subtilisin-like proprotein convertase family. The FUR gene is located approximately 1 kb upstream of the FES gene on chromosome 15q25-q26. This gene is thought to play a role in tumor progression. Furin is one of the proteases responsible for the proteolytic cleavage of HIV envelope polyprotein precursor gp160 to gp120 and gp41 prior to viral assembly. Furin is enriched in the Golgi apparatus, where it functions to cleave other proteins into their mature/active forms. Expression of furin in T-cells is required for maintenance of peripheral immune tolerance
Uniprot IDP09958
Protein Accession #NP_001276752.1
Nucleotide Accession #NM_001289823.1
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