FTH1 ELISA Kit (Mouse) (OKCD00365)

Catalog No: OKCD00365 (Formerly GWB-KBBRM5)

Size:96WELLS

Mouse Fth1 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Mouse|Mus musculus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2 Dilution	1:4 Dilution	1:8 Dilution	1:16 Dilution
  serum(n=5)	92-103%	83-103%	92-101%	80-101%
  EDTA plasma(n=5)	82-101%	88-97%	87-98%	89-97%
  heparin plasma(n=5)	91-105%	95-102%	80-90%	86-101%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Ferritin, Heavy Polypeptide (FTH). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Ferritin, Heavy Polypeptide (FTH). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Ferritin, Heavy Polypeptide (FTH), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Ferritin, Heavy Polypeptide (FTH) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 15.6-1,000pg/mL
• ELISA Kit Recovery:

  Matrix	Recovery Range (%)	Average (%)
  serum(n=5)	98-105	101
  EDTA plasma(n=5)	97-105	101
  heparin plasma(n=5)	93-104	101

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Ferritin, Heavy Polypeptide (FTH) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Ferritin, Heavy Polypeptide (FTH) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-Fth1 Microplate: 96 Wells (12 x 8 Well Strips)
  • Fth1 Lyophilized Standard: 2 x 10 ng
  • 100X Biotinylated Fth1 Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Sensitivity: < 0.057 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Ferritin, Heavy Polypeptide (FTH). No significant cross-reactivity or interference between Ferritin, Heavy Polypeptide (FTH) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: Fth1
• Gene Full Name: ferritin heavy polypeptide 1
• Alias Symbols: ferritin H subunit;ferritin heavy chain;ferritin heavy chain 1;FHC;Fth;H-fer;H-ferritin;HFt;MFH.
• NCBI Gene Id: 14319
• Protein Name: Ferritin heavy chain
• Description of Target: Stores iron in a soluble, non-toxic, readily available form. Important for iron homeostasis. Has ferroxidase activity. Iron is taken up in the ferrous form and deposited as ferric hydroxides after oxidation. Also plays a role in delivery of iron to cells. Mediates iron uptake in capsule cells of the developing kidney.
• Uniprot ID: P09528
• Protein Accession #: NP_034369
• Nucleotide Accession #: NM_010239.2
• Protein Size (# AA): 182