FSHR ELISA Kit (Human) (OKCD02561)

Catalog No: OKCD02561

Size:96 Wells

Human FSHR ELISA kit standard curve

Product Info

• Predicted Species Reactivity: Human
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: 3 Hours
• ELISA Kit Principle: Aviva Systems Biology FSHR ELISA Kit (Human) (OKCD02561) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for FSHR has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wellss, incubated and removed.A biotinylated detector antibody specific for FSHR is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample FSHR captured in well.
• ELISA Kit Range: 0.312-20 ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision: 3 samples with known low, middle and high level FSHR was tested with 20 replicates across one plate.
  Inter-assay Precision: 3 samples with known low, middle and high level FSHR was tested on three different plates with 8 replicates, respectively.
  Intra-Assay CV: <10% (n=20)
  Inter-Assay CV: <12% (n=24)
• ELISA Kit Component:
  • Anti-FSHR Microplate: 96 Wells (12 x 8 Well strips)
  • FSHR Lyophilized Standard: 2
  • 100X Biotinylated FSHR Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • Stop Solution: 1 x 6 mL
  • TMB Substrate: 1 x 9 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Tissue homogenates, cell lysates and other biological fluids
• Sensitivity: 0.124 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Follicle Stimulating Hormone Receptor (FSHR). No significant cross-reactivity or interference between Follicle Stimulating Hormone Receptor (FSHR) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: FSHR
• Gene Full Name: follicle stimulating hormone receptor
• Alias Symbols: LGR1, ODG1, FSHR1, FSHRO
• NCBI Gene Id: 2492
• Protein Name: Follicle-stimulating hormone receptor
• Description of Target: Receptor for follicle-stimulating hormone. The activity of this receptor is mediated by G proteins which activate adenylate cyclase. Induces cAMP production through the activation of PI3K-AKT and SRC-ERK1/2 signaling pathways.
• Uniprot ID: P23945
• Protein Accession #: NP_000136.2
• Nucleotide Accession #: NM_000145.3
• Protein Size (# AA): 695