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Catalog No: OKCA00138
Size:96 Wells
Price: $475.00
SKU
OKCA00138
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Please click here to view the MSDS.
COA DatasheetPrintable COA datasheet for OKCA00138
Product Info
Predicted Species ReactivityMouse
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration< 4 Hours
ELISA Kit PrincipleAviva Systems Biology Free Tri-iodothyronine ELISA Kit (Mouse) (OKCA00138) is based on a competitive enzyme immunoassay technique. The microtiter well-plate in this kit has been pre-coated with an anti-mouse Free Tri-iodothyronine antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated Free Tri-iodothyronine and incubated. The Free Tri-iodothyronine found in the sample or standards competes with the biotinylated Free Tri-iodothyronine for limited binding sites on the immobilized anti-mouse Free Tri-iodothyronine antibody. Excess unbound biotinylated Free Tri-iodothyronine and sample or standard Free Tri-iodothyronine is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated Free Tri-iodothyronine captured in the well and inversely proportional to the amount of Free Tri-iodothyronine which was contained in the sample or standard.
ELISA Kit Range2 pmol/l - 32 pmol/l
ELISA Kit Reproducibility
Mean Intra-Assay Precision %CVMean Inter-Assay Precision %CV
Sample123123
n202020202020
Mean (pmol/L)5.3395.4285.3875.3515.4865.48
SD0.0410.0420.0390.0470.0460.045
CV%2.9433.0642.8163.43.4023.322
ELISA Kit Component
ComponentAmount
Anti- Free Tri-iodothyronine Microplate96 Well (12 x 8 Well Strips)
Free Tri-iodothyronine Standard 5 x 1 mL
Free Tri-iodothyronine Antibody-Biotin Complex1 x 6 mL
Avidin-HRP Conjugate1 x 6 mL
20X Wash Buffer1 x 15 mL
Substrate A1 x 7 mL
Substrate B1 x 7 mL
Stop Solution1 x 7 mL
Additional Information

CAS#: 6893-02-3

Molecular Formula: C15H12I3NO4

Molecular Weight: 650.9776 g mol−1

Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Cell culture supernates and Tissue homogenates
Sensitivity< 0.38 pmoL/L
SpecificityThis assay has high sensitivity and excellent specificity for detection of mouse Free-T3. No significant cross-reactivity or interference between mouse Free-T3
and analogues was observed.
Assay InfoAssay Methodology: Quantitative Competitive Immunoassay
Description
Gene SymbolFree Tri-iodothyronine
Alias Symbols3,3',5-Triiodo-L-thyronine, 3,3',5-Triiodothyronine, 3,5,3'-Triiodothyronine, 4-(4-Hydroxy-3-iodophenoxy)-3,5-diiodophenylalanine, Alanine, 3-[4-(4-hydroxy-3-iodophenoxy)-3,5-diiodophenyl]-, L-Cyronine, L-3,3',5-Triiodothyronine, othyronin, Liothyronin, l
Protein NameFree Tri-iodothyronine
Description of TargetAn iodothyronine compound having iodo substituents at the 3-, 3'- and 5-positions. Although some is produced in the thyroid, most of the 3,3',5-triiodo-L-thyronine in the body is generated by mono-deiodination of L-thyroxine in the peripheral tissues. Its metabolic activity is about 3 to 5 times that of L-thyroxine. The sodium salt is used in the treatment of hypothyroidism.
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