Free-Thyroxine 4 ELISA Kit (Mouse) (OKCA00139)

Catalog No: OKCA00139

Size:96 Wells

Mouse Free-Thyroxine 4 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Mouse
• ELISA Kit Detection Method: Colorimetric, OD 450 nm
• ELISA Kit Duration: < 4 Hours
• ELISA Kit Principle: Aviva Systems Biology Free-Thyroxine 4 ELISA Kit (Mouse) (OKCA00139) is based on a competitive enzyme immunoassay technique. The microtiter well-plate in this kit has been pre-coated with an anti-mouse Free-Thyroxine 4 antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated Free-Thyroxine 4 and incubated. The Free-Thyroxine 4 found in the sample or standards competes with the biotinylated Free-Thyroxine 4 for limited binding sites on the immobilized anti-mouse Free-Thyroxine 4 antibody. Excess unbound biotinylated Free-Thyroxine 4 and sample or standard Free-Thyroxine 4 is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated Free-Thyroxine 4 captured in the well and inversely proportional to the amount of Free-Thyroxine 4 which was contained in the sample or standard.
• ELISA Kit Range: 4 pmol/l - 64 pmol/l
• ELISA Kit Reproducibility:

  	Mean Intra-Assay Precision %CV			Mean Inter-Assay Precision %CV
  Sample	1	2	3	1	2	3
  n	20	20	20	20	20	20
  Mean (pmolL	16.566	16.625	16.726	16.66	16.47	16.597
  SD	0.037	0.04	0.033	0.049	0.052	0.056
  CV%	4.501	4.868	4.048	5.948	6.263	6.887

• ELISA Kit Component:
  • Anti- Free-Thyroxine 4 Microplate: 96 Well (12 x 8 Well Strips)
  • Free-Thyroxine 4 Standard: 5 x 1 mL
  • Free-Thyroxine 4-Biotin Complex: 1 x 6 mL
  • Avidin-HRP Conjugate: 1 x 6 mL
  • 20X Wash Buffer: 1 x 15 mL
  • Substrate A: 1 x 7 mL
  • Substrate B: 1 x 7 mL
  • Stop Solution: 1 x 7 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue homogenates
• Sensitivity: 1.08 pmol/L
• Specificity: This assay has high sensitivity and excellent specificity for detection of mouse FT4. No significant cross-reactivity or interference between mouse FT4 and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Competitive ELISA
• Description:
  View product citations for Free-Thyroxine 4 ELISA Kit (Mouse) (OKCA00139) on CiteAb

Target Info

• Gene Symbol: Free-Thyroxine 4
• Alias Symbols: T4, Thyroxine, T4
• Protein Name: Free-Thyroxine 4