Catalog No: OKDD00274 (Formerly GWB-KBBYN6)
Size:96 Wells
Price: $750.00
SKU
OKDD00274
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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- Toll Free: 888-880-0001
- Phone: 858-552-6979
- Email: info@avivasysbio.com
Shipping Info:
- $55: Antibody & Protein in US
- $55 + $25/Kit in US
- Contact us for international orders.
Datasheets/Manuals | Printable datasheet for FPR1 ELISA Kit (Human) (OKDD00274) |
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Predicted Species Reactivity | Homo sapiens|Human | ||||||||||||||||||||||||
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Application | Enzyme-linked immunosorbent assay | ||||||||||||||||||||||||
ELISA Kit Detection Method | Colorimetric, OD450 nm | ||||||||||||||||||||||||
ELISA Kit Duration | ~4 Hours | ||||||||||||||||||||||||
ELISA Kit Principle | Aviva Systems Biology FPR1 ELISA Kit (Human) (OKDD00274) is based on standard sandwich enzyme-linked immuno-sorbent assay methodology. An antibody for FPR1 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for FPR1 is added, incubated and removed. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample FPR1 captured in well. | ||||||||||||||||||||||||
ELISA Kit Range | 0.156-10ng/mL | ||||||||||||||||||||||||
ELISA Kit Reproducibility | Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level FPR1 were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level FPR1 were tested on 3 different plates, 8 replicates in each plate. Intra-Assay: CV<10%
Inter-Assay: CV<12% | ||||||||||||||||||||||||
ELISA Kit Component |
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Reconstitution and Storage | Store as indicated in product manual. | ||||||||||||||||||||||||
Sample Type | Cell lysates, Cell culture supernatants and other biological fluids | ||||||||||||||||||||||||
Sensitivity | < 0.062 ng/mL | ||||||||||||||||||||||||
Specificity | This assay has high sensitivity and excellent specificity for detection of FPR1. No significant cross-reactivity or interference between FPR1 and analogues was observed. | ||||||||||||||||||||||||
Assay Info | Quantitative Sandwich ELISA |
Gene Symbol | FPR1 |
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Gene Full Name | formyl peptide receptor 1 |
Alias Symbols | fMet-Leu-Phe receptor;FMLP;fMLP receptor;FPR;N-formyl peptide receptor;N-formylpeptide chemoattractant receptor. |
NCBI Gene Id | 2357 |
Protein Name | fMet-Leu-Phe receptor |
Description of Target | High affinity receptor for N-formyl-methionyl peptides (fMLP), which are powerful neutrophil chemotactic factors (PubMed:2161213, PubMed:2176894, PubMed:10514456, PubMed:15153520). Binding of fMLP to the receptor stimulates intracellular calcium mobilization and superoxide anion release (PubMed:2161213, PubMed:1712023, PubMed:15153520). This response is mediated via a G-protein that activates a phosphatidylinositol-calcium second messenger system (PubMed:1712023, PubMed:10514456). |
Uniprot ID | P21462 |
Protein Accession # | NP_001180235.1 |
Nucleotide Accession # | NM_001193306.1 |
Protein Size (# AA) | 350 |
Molecular Weight | 38 kDa |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
-
See our General FAQ page.
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