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Catalog No: OKDD00274 (Formerly GWB-KBBYN6)
Size:96 Wells
Price: $750.00
SKU
OKDD00274
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Datasheets/ManualsPrintable datasheet for FPR1 ELISA Kit (Human) (OKDD00274)
Product Info
Predicted Species ReactivityHomo sapiens|Human
ApplicationEnzyme-linked immunosorbent assay
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~4 Hours
ELISA Kit PrincipleAviva Systems Biology FPR1 ELISA Kit (Human) (OKDD00274) is based on standard sandwich enzyme-linked immuno-sorbent assay methodology. An antibody for FPR1 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for FPR1 is added, incubated and removed. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample FPR1 captured in well.
ELISA Kit Range0.156-10ng/mL
ELISA Kit Reproducibility

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level FPR1 were tested 20 times on one plate, respectively.

Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level FPR1 were tested on 3 different plates, 8 replicates in each plate.

Intra-Assay: CV<10%

Inter-Assay: CV<12%

ELISA Kit Component
ComponentAmount
Anti-FPR1 Microplate96 Wells (12 x 8 Well Strips)
FPR1 Lyophlized Standard2 x 10 ng
Standard Diluent1 x 20 mL
100X FPR1 Biotinylated Detector Antibody1 x 120 uL
100X Avidin-HRP Detection Conjugate1 x 120 uL
Detector Antibody Diluent1 x 12 mL
Detection Conjugate Diluent1 x 12 mL
10X Sample Diluent1 x 30 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeCell lysates, Cell culture supernatants and other biological fluids
Sensitivity< 0.062 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of FPR1. No significant cross-reactivity or interference between FPR1 and analogues was observed.
Assay InfoQuantitative Sandwich ELISA
Gene SymbolFPR1
Gene Full Nameformyl peptide receptor 1
Alias SymbolsfMet-Leu-Phe receptor;FMLP;fMLP receptor;FPR;N-formyl peptide receptor;N-formylpeptide chemoattractant receptor.
NCBI Gene Id2357
Protein NamefMet-Leu-Phe receptor
Description of TargetHigh affinity receptor for N-formyl-methionyl peptides (fMLP), which are powerful neutrophil chemotactic factors (PubMed:2161213, PubMed:2176894, PubMed:10514456, PubMed:15153520). Binding of fMLP to the receptor stimulates intracellular calcium mobilization and superoxide anion release (PubMed:2161213, PubMed:1712023, PubMed:15153520). This response is mediated via a G-protein that activates a phosphatidylinositol-calcium second messenger system (PubMed:1712023, PubMed:10514456).
Uniprot IDP21462
Protein Accession #NP_001180235.1
Nucleotide Accession #NM_001193306.1
Protein Size (# AA)350
Molecular Weight38 kDa
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