FOXA2 antibody - middle region (ARP31944_P050)

Catalog#: ARP31944_P050

• Size: 50ug

Human HeLa

Human Lung

• Description of Target: FOXA2 is a member of the forkhead class of DNA-binding proteins. These hepatocyte nuclear factors are transcriptional activators for liver-specific transcripts such as albumin and transthyretin, and they also interact with chromatin. Similar family members in mice have roles in the regulation of metabolism and in the differentiation of the pancreas and liver. This gene has been linked to sporadic cases of maturity-onset diabetes of the young.This gene encodes a member of the forkhead class of DNA-binding proteins. These hepatocyte nuclear factors are transcriptional activators for liver-specific transcripts such as albumin and transthyretin, and they also interact with chromatin. Similar family members in mice have roles in the regulation of metabolism and in the differentiation of the pancreas and liver. This gene has been linked to sporadic cases of maturity-onset diabetes of the young. Two transcript variants encoding the same protein have been identified for this gene. This gene encodes a member of the forkhead class of DNA-binding proteins. These hepatocyte nuclear factors are transcriptional activators for liver-specific transcripts such as albumin and transthyretin, and they also interact with chromatin. Similar family members in mice have roles in the regulation of metabolism and in the differentiation of the pancreas and liver. This gene has been linked to sporadic cases of maturity-onset diabetes of the young. Two transcript variants encoding the same protein have been identified for this gene.
• Gene Symbol: FOXA2
• Official Gene Full Name: Forkhead box A2
• Alias Symbols: HNF3B; MGC19807; TCF3B
• Protein Accession#: NP_068556
• Nucleotide Accession#: NM_021784
• Swissprot Id: Q9Y261
• Host: Rabbit
• Clonality: Polyclonal
• Protein Size (# AA): 457
• Molecular Weight: 48kDa
• Application: WB
• Partner Proteins: HMGB1
• Immunogen: The immunogen for anti-FOXA2 antibody: synthetic peptide directed towards the middle region of human FOXA2
• Product Format: Lyophilized powder
• Purification: Affinity Purified
• Predicted Homology Based on Immunogen Sequence: Dog: 100%; Horse: 100%; Human: 100%; Pig: 100%; Mouse: 92%; Rat: 91%
• Peptide Sequence: Synthetic peptide located within the following region: ASQAQLGEAAGPASETPAGTESPHSSASPCQEHKRGGLGELKGTPAAALS
• Blocking Peptide: For anti-FOXA2 antibody is Catalog # AAP31944 (Previous Catalog # AAPP02841)
• Key Reference: Yang,G., (2008) Blood 111 (1), 392-401
• Reconstitution and Storage: Add 50 μl of distilled water. Final anti-FOXA2 antibody concentration is 1 mg/ml in PBS buffer. For longer periods of storage, store at -20°C. Avoid repeat freeze-thaw cycles.

Product Protocols: FOXA2 antibody tested with Human Hela Cells (ARP31944_P050)

Aviva Systems Biology is the original manufacturer of this FOXA2 antibody (ARP31944_P050)

Click here to view the FOXA2 antibody Western Blot Protocol

Product Datasheet Link: FOXA2 antibody (ARP31944_P050)

WB Suggested Anti-FOXA2 Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1:12500
Positive Control: Hela

Western Blot image:


Description of Target: FOXA2 is a member of the forkhead class of DNA-binding proteins. These hepatocyte nuclear factors are transcriptional activators for liver-specific transcripts such as albumin and transthyretin, and they also interact with chromatin. Similar family members in mice have roles in the regulation of metabolism and in the differentiation of the pancreas and liver. This gene has been linked to sporadic cases of maturity-onset diabetes of the young.This gene encodes a member of the forkhead class of DNA-binding proteins. These hepatocyte nuclear factors are transcriptional activators for liver-specific transcripts such as albumin and transthyretin, and they also interact with chromatin. Similar family members in mice have roles in the regulation of metabolism and in the differentiation of the pancreas and liver. This gene has been linked to sporadic cases of maturity-onset diabetes of the young. Two transcript variants encoding the same protein have been identified for this gene. This gene encodes a member of the forkhead class of DNA-binding proteins. These hepatocyte nuclear factors are transcriptional activators for liver-specific transcripts such as albumin and transthyretin, and they also interact with chromatin. Similar family members in mice have roles in the regulation of metabolism and in the differentiation of the pancreas and liver. This gene has been linked to sporadic cases of maturity-onset diabetes of the young. Two transcript variants encoding the same protein have been identified for this gene.

Questions pertaining to this data can be directed to techsupport@avivasysbio.com

Aviva Systems Biology’s FOXA2 antibody (ARP31944_P050) has been tested using other cell lysates and tissues. To obtain more data about this antibody please email us at info@avivasysbio.com.

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Product Review: FOXA2 antibody - middle region (ARP31944_P050) in Human lung tissue using Western Blot

Product Page Link: FOXA2 antibody - middle region (ARP31944_P050)

Data Provided By Dr. Yingze Zhang, University of Pittsburgh

Protocols and Procedures may be found in this publication:

Am J Physiol Lung Cell Mol Physiol. 2012 Jan 20. [Epub ahead of print]

Allele Specific Transactivation of Matrix Metalloproteinase 7 by FOXA2 and Correlation with Plasma Levels in Idiopathic Pulmonary Fibrosis.

Richards T, Park C, Chen Y, Gibson KF, Di YP, Pardo A, Watkins SC, Choi AM, Selman M, Pilewski JM, Kaminski N, Zhang Y.

Source

1University of Pittsburgh.

Abstract

Idiopathic pulmonary fibrosis (IPF) is a complex disease with poorly understood etiology. Previously we reported up-regulation of matrix metalloproteinase 7 (MMP7) in both lung and peripheral blood of IPF patients. Here we report evidence for genetic correlation of plasma levels and promoter polymorphisms (rs11568818 and rs11568819) of MMP7 in a well-characterized IPF cohort. Both the AA genotype of rs11568818 and the CT genotype of rs11568819 were found to be significantly associated with higher MMP7 plasma levels. These associations were observed only in IPF patients and not in healthy controls. The G-to-A transition of rs11568818 resulted in a novel binding site for the forkhead box A2 (FOXA2) transcription factor, a key regulator of embryonic lung development and proper function of the mature lung. In vitro, this transition led to increased sensitivity of the MMP7 promoter to FOXA2. In IPF lungs, FOXA2 was localized in the nucleus of epithelial cells that expressed MMP7 in the cytoplasm. These results suggest that increased sensitivity of the polymorphic MMP7 promoter to FOXA2 provides one of the genetic bases for the up-regulation of MMP7 in IPF.