Catalog No: OKEH04767
Size:96T
Price: $525.00
SKU
OKEH04767
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHomo sapiens, Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~3 Hours
ELISA Kit PrincipleAviva Systems Biology FOPNL ELISA Kit (Human) (OKEH04767) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for FOPNL has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for FOPNL is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample FOPNL captured in well.
ELISA Kit Range0.312-20ng/ml
ELISA Kit ReproducibilityMean Intra-Assay CV: < 10%
Mean Inter-Assay CV: < 12%
ELISA Kit Component
ComponentAmount
FOPNL Microplate96 Wells (12 x 8 Well Strips)
FOPNL Standard2
Sample Diluent1 x 20 mL
FOPNL Biotinylated Detector Antibody1 x 120 uL
Detector Antibody Diluent1 x 12 mL
HRP Avidin Conjugate1 x 120 uL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue homogenates, Cell culture supernates and Other biological fluids.
Sensitivity0.174 ng/mL
SpecificityNatural and recombinant Human LisH domain-containing protein FOPNL
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolCEP20
Gene Full Namecentrosomal protein 20
Alias SymbolsC16orf63;centrosomal protein 20;FGFR1OP N-terminal like;FGFR1OP N-terminal-like protein;FOPNL;FOP-related protein of 20 kDa;FOR20;lisH domain-containing protein C16orf63;lisH domain-containing protein FOPNL;PHSECRG2;pluripotent embryonic stem cell-related protein.
NCBI Gene Id123811
Protein NameQ96NB1
Description of TargetLisH domain-containing protein FOPNL
Uniprot IDhttps://www.uniprot.org/uniprot/Q96NB1
Protein Accession #NP_001291426.1
Nucleotide Accession #NM_001304497.1
Protein Size (# AA)174
Molecular Weight20 kDa
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