FNTA ELISA Kit (Human) (OKCD00703)

Catalog No: OKCD00703 (Formerly GWB-KBBPQ2)

Size:96WELLS

Human FNTA ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2 Dilution	1:4 Dilution	1:8 Dilution	1:16 Dilution
  serum(n=5)	87-101%	80-95%	78-97%	92-101%
  EDTA plasma(n=5)	92-105%	78-95%	85-99%	91-99%
  heparin plasma(n=5)	78-104%	79-102%	78-104%	78-92%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Farnesyltransferase Alpha (FNTa). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Farnesyltransferase Alpha (FNTa). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Farnesyltransferase Alpha (FNTa), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Farnesyltransferase Alpha (FNTa) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Recovery:

  Matrix	Recovery Range (%)	Average (%)
  serum(n=5)	80-99	96
  EDTA plasma(n=5)	79-102	84
  heparin plasma(n=5)	88-96	93

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Farnesyltransferase Alpha (FNTa) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Farnesyltransferase Alpha (FNTa) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-FNTA Microplate: 96 Wells (12 x 8 Well Strips)
  • FNTA Lyophilized Standard: 2 x 100 ng
  • 100X Biotinylated FNTA Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, tissue homogenates and other biological fluids.
• Sensitivity: < 0.56 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Farnesyltransferase Alpha (FNTa). No significant cross-reactivity or interference between Farnesyltransferase Alpha (FNTa) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: FNTA
• Gene Full Name: farnesyltransferase, CAAX box, alpha
• Alias Symbols: CAAX farnesyltransferase subunit alpha;farnesyl-protein transferase alpha-subunit;FPTA;FTase-alpha;GGTase-I-alpha;PGGT1A;protein farnesyltransferase/geranylgeranyltransferase type-1 subunit alpha;protein prenyltransferase alpha subunit repeat containing 2;PTAR2;ras proteins prenyltransferase subunit alpha;type I protein geranyl-geranyltransferase alpha subunit;Type I protein geranyl-geranyltransferase subunit alpha.
• NCBI Gene Id: 2339
• Protein Name: Protein farnesyltransferase/geranylgeranyltransferase type-1 subunit alpha
• Description of Target: Essential subunit of both the farnesyltransferase and the geranylgeranyltransferase complex. Contributes to the transfer of a farnesyl or geranylgeranyl moiety from farnesyl or geranylgeranyl diphosphate to a cysteine at the fourth position from the C-terminus of several proteins having the C-terminal sequence Cys-aliphatic-aliphatic-X. May positively regulate neuromuscular junction development downstream of MUSK via its function in RAC1 prenylation and activation.
• Uniprot ID: P49354
• Protein Accession #: NP_002018
• Nucleotide Accession #: NM_002027.2
• Protein Size (# AA): 379