Catalog No: OKEH04551
Size:96T
Price: $525.00
SKU
OKEH04551
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
Please contact customer service when ordering 10+ kits.
Contact Us:
- Toll Free: 888-880-0001
- Phone: 858-552-6979
- Email: info@avivasysbio.com
Shipping Info:
- $55: Antibody & Protein in US
- $55 + $25/Kit in US
- Contact us for international orders.
Datasheets/Manuals | Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit. |
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Predicted Species Reactivity | Rat, Rattus norvegicus | ||||||||||||||||||||||
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Application | Enzyme-linked Immunosorbent assay-Sandwich | ||||||||||||||||||||||
ELISA Kit Detection Method | Colorimetric, OD450 nm | ||||||||||||||||||||||
ELISA Kit Duration | ~3 Hours | ||||||||||||||||||||||
ELISA Kit Principle | Aviva Systems Biology Flt4 ELISA Kit (Rat) (OKEH04551) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Flt4 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for Flt4 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Flt4 captured in well. | ||||||||||||||||||||||
ELISA Kit Range | 0.156-10ng/mL | ||||||||||||||||||||||
ELISA Kit Reproducibility | Mean Intra-Assay CV: < 10% Mean Inter-Assay CV: < 12% | ||||||||||||||||||||||
ELISA Kit Component |
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Reconstitution and Storage | Store as indicated in product manual. | ||||||||||||||||||||||
Sample Type | serum, Plasma, Urine, tissue homogenates and Cell culture supernates and Other biological fluids | ||||||||||||||||||||||
Sensitivity | 0.078 ng/mL | ||||||||||||||||||||||
Specificity | Natural and recombinant Rat Vascular endothelial growth factor receptor 3 | ||||||||||||||||||||||
Assay Info | Assay Methodology: Quantitative Sandwich ELISA |
Gene Symbol | Flt4 |
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Gene Full Name | Fms related receptor tyrosine kinase 4 |
Alias Symbols | FLT-4;FMS-like tyrosine kinase 4;fms-related tyrosine kinase 4;tyrosine-protein kinase receptor FLT4;vascular endothelial growth factor receptor 3;Vegfr3;VEGFR-3. |
NCBI Gene Id | 114110 |
Protein Name | Q91ZT1 |
Description of Target | Vascular endothelial growth factor receptor 3 |
Uniprot ID | https://www.uniprot.org/uniprot/Q91ZT1 |
Protein Accession # | NP_446104.1 |
Nucleotide Accession # | NM_053652.1 |
Protein Size (# AA) | 1363 |
Molecular Weight | 153 KDa |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
-
See our General FAQ page.
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