FLT3 ELISA Kit (Human) : 96 Wells (OKEH00059)

Catalog No: OKEH00059

Size:96T

Human FLT3 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens, Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: Aviva Systems Biology FLT3 ELISA Kit (Human) (OKEH00059) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for FLT3 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for FLT3 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample FLT3 captured in well.
• ELISA Kit Range: 78-5000pg/mL
• ELISA Kit Recovery: Mean recovery when spiking into Serum and Plasma = 93%
• ELISA Kit Reproducibility: Mean Intra-assay CV%: < 3.9% (n = 20)
  Mean Inter-assay CV%: < 6.2% (n = 20)
• ELISA Kit Component:
  • FLT3 Microplate: 96 Wells (12 x 8 Well strips)
  • FLT3 Lyophilized Standard: 2
  • 100X Biotinylated FLT3 Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Urine, Tissue homogenates, Cell culture supernates, Other biological fluids
• Sensitivity: 21 pg/mL
• Specificity: Natural and recombinant Human Receptor-type tyrosine-protein kinase FLT3
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: FLT3
• Gene Full Name: fms related receptor tyrosine kinase 3
• Alias Symbols: CD135;CD135 antigen;fetal liver kinase 2;Fetal liver kinase-2;FL cytokine receptor;FLK2;FLK-2;fms related tyrosine kinase 3;fms-like tyrosine kinase 3;growth factor receptor tyrosine kinase type III;receptor-type tyrosine-protein kinase FLT3;stem cell tyrosine kinase 1;STK1.
• NCBI Gene Id: 2322
• Protein Name: P36888
• Description of Target: Receptor-type tyrosine-protein kinase FLT3
• Uniprot ID: https://www.uniprot.org/uniprot/P36888
• Protein Accession #: NP_004110
• Nucleotide Accession #: NM_004119
• Protein Size (# AA): 993
• Molecular Weight: 113 kDa