Catalog No: OKBB00395
Size:96 Wells
Price: $583.00
SKU
OKBB00395
Availability: Domestic: within 1-2 week delivery | International: within 1-2 week delivery
Contact Us:
- Toll Free: 888-880-0001
- Phone: 858-552-6979
- Email: info@avivasysbio.com
Shipping Info:
- $55: Antibody & Protein in US
- $55 + $25/Kit in US
- Contact us for international orders.
Datasheets/Manuals | Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit. |
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Predicted Species Reactivity | Mouse | ||||||||||||||||||||||||||||||||||||||||||
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Application | ELISA | ||||||||||||||||||||||||||||||||||||||||||
ELISA Kit Detection Method | Colorimetric, OD450 nm | ||||||||||||||||||||||||||||||||||||||||||
ELISA Kit Duration | 2.5 Hours | ||||||||||||||||||||||||||||||||||||||||||
ELISA Kit Principle | Aviva's mouse Flt-3ligand ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for Flt-3ligand has been precoated onto 96-well plates. Standards(NS0, G27-R188) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Flt-3ligand is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the mouse Flt-3ligand amount of sample captured in plate. | ||||||||||||||||||||||||||||||||||||||||||
ELISA Kit Range | 31.2 pg/ml - 2,000 pg/ml | ||||||||||||||||||||||||||||||||||||||||||
ELISA Kit Reproducibility |
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ELISA Kit Component |
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Reconstitution and Storage | Store at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.) | ||||||||||||||||||||||||||||||||||||||||||
Sample Type | Cell culture supernatants and serum | ||||||||||||||||||||||||||||||||||||||||||
Sensitivity | <10 pg/ml | ||||||||||||||||||||||||||||||||||||||||||
Specificity | Natural and recombinant mouse Flt-3ligand | ||||||||||||||||||||||||||||||||||||||||||
Assay Info | Assay Methodology: Quantitative Sandwich Immunoassay | ||||||||||||||||||||||||||||||||||||||||||
Immunogen | Expression system for standard: NS0; Immunogen sequence: G27-R188 | ||||||||||||||||||||||||||||||||||||||||||
Description | For quantitative detection of mouse Flt-3ligand in cell culture supernatants and serum. |
Gene Symbol | FLT3LG |
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Gene Full Name | fms-related tyrosine kinase 3 ligand |
Alias Symbols | Flt 3 ligand, Flt 3L, Flt3 L, FLT3 LG, Flt3 ligand, Flt3L, FLT3L_HUMAN, Flt3lg, Fms related tyrosine kinase 3 ligand, Fms-related tyrosine kinase 3 ligand, SL cytokine |
NCBI Gene Id | 14256 |
Protein Name | Fms-related tyrosine kinase 3 ligand |
Description of Target | Fms-related tyrosine kinase 3 ligand (FLT3LG), also known as Flt-3ligand or FL, is a protein which in humans is encoded by the FLT3LG gene. It is mapped to 19q13.3. Flt-3ligand is a hematopoietic four helical bundle cytokine. It is structurally homologous to stem cell factor (SCF) and colony stimulating factor 1 (CSF-1). In synergy with other growth factors, Flt-3ligand stimulates the proliferation and differentiation of various blood cell progenitors. Besides that, Flt-3ligand can control the development of Dendritic cells (DCs) and is particularly important for plasmacytoid DCs and CD8-positive classical DCs and their CD103-positive tissue counterparts. Flt-3ligand also can enhance the response of stem and primitive progenitor cells to other growth factors to generate all myeloid lineages except erythroid cells. |
Uniprot ID | P49772 |
Protein Accession # | NP_038548.3 |
Nucleotide Accession # | NM_013520.3 |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
-
See our General FAQ page.
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