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Catalog No: OKBB00395
Size:96 Wells
Price: $583.00
SKU
OKBB00395
Availability: Domestic: within 1-2 week delivery | International: within 1-2 week delivery
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityMouse
ApplicationELISA
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration2.5 Hours
ELISA Kit PrincipleAviva's mouse Flt-3ligand ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for Flt-3ligand has been precoated onto 96-well plates. Standards(NS0, G27-R188) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Flt-3ligand is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the mouse Flt-3ligand amount of sample captured in plate.
ELISA Kit Range31.2 pg/ml - 2,000 pg/ml
ELISA Kit Reproducibility
Intra-Assay PrecisionInter-Assay Precision
Sample123123
n 161616242424
Mean (pg/mL)20755613242125451318
SD11.1830.0259.5817.8136.5286.99
CV%5.45.44.58.46.76.6
ELISA Kit Component
ComponentAmount
96-Well plate Pre-coated with Anti-Mouse Flt-3 Ligand Antibody1 x (12 x 8 Well Strip)
Lyophilized Recombinant Mouse Flt-3 Ligand Standard2 x 10 ng/tube
100X Biotinylated Anti-Mouse Flt-3 Ligand Antibody1 x 130 uL
100X Avidin-Biotin-Peroxidase Complex (ABC)1 x 130 uL
Sample Diluent Buffer1 x 30 mL
Antibody Diluent Buffer1 x 12 mL
ABC Diluent Buffer1 x 12 mL
TMB Color Developing Agent1 x 10 mL
TMB Stop Solution1 x 10 mL
10X Wash Buffer1 x 30 mL
Reconstitution and StorageStore at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Sample TypeCell culture supernatants and serum
Sensitivity<10 pg/ml
SpecificityNatural and recombinant mouse Flt-3ligand
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
ImmunogenExpression system for standard: NS0; Immunogen sequence: G27-R188
DescriptionFor quantitative detection of mouse Flt-3ligand in cell culture supernatants and serum.
Gene SymbolFLT3LG
Gene Full Namefms-related tyrosine kinase 3 ligand
Alias SymbolsFlt 3 ligand, Flt 3L, Flt3 L, FLT3 LG, Flt3 ligand, Flt3L, FLT3L_HUMAN, Flt3lg, Fms related tyrosine kinase 3 ligand, Fms-related tyrosine kinase 3 ligand, SL cytokine
NCBI Gene Id14256
Protein NameFms-related tyrosine kinase 3 ligand
Description of TargetFms-related tyrosine kinase 3 ligand (FLT3LG), also known as Flt-3ligand or FL, is a protein which in humans is encoded by the FLT3LG gene. It is mapped to 19q13.3. Flt-3ligand is a hematopoietic four helical bundle cytokine. It is structurally homologous to stem cell factor (SCF) and colony stimulating factor 1 (CSF-1). In synergy with other growth factors, Flt-3ligand stimulates the proliferation and differentiation of various blood cell progenitors. Besides that, Flt-3ligand can control the development of Dendritic cells (DCs) and is particularly important for plasmacytoid DCs and CD8-positive classical DCs and their CD103-positive tissue counterparts. Flt-3ligand also can enhance the response of stem and primitive progenitor cells to other growth factors to generate all myeloid lineages except erythroid cells.
Uniprot IDP49772
Protein Accession #NP_038548.3
Nucleotide Accession #NM_013520.3
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