FLRT2 ELISA Kit (Human) (OKCA01329)

Catalog No: OKCA01329

Size:96 Wells

Human FLRT2 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Human
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3.5 Hours
• ELISA Kit Linearity:

  Dilution	Mean Recovery (%)	Range (%)
  Neat	85	80-90
  1:2	95	90-100
  1:4	106	102-114
  1:8	89	84-93

• ELISA Kit Principle: Aviva Systems Biology FLRT2 ELISA Kit (Human) (OKCA01329) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for FLRT2 has been pre-coated onto a 96-well plate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for FLRT2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample FLRT2 captured in the well.
• ELISA Kit Range: 93.75 - 6,000 pg/mL
• ELISA Kit Recovery:

  Sample	Mean Recovery (%)	Range (%)
  Serum (n=5)	98	94-102
  EDTA Plasma (n=4)	92	88-96

• ELISA Kit Reproducibility: Intra-assay Precision: 3 samples with known low, middle and high level OKCA01329 were tested with 20 replicates across one plate.
  Inter-assay Precision: 3 samples with known low, middle and high level OKCA01329 were tested on three different plates with 8 replicates, respectively.
  Intra-Assay CV: <8%
  Inter-Assay CV: <10%
• ELISA Kit Component:
  • FLRT2 Microplate: 96 Wells (12 x 8 Well strips)
  • FLRT2 Lyophilized Standard: 2 x 6 ng
  • 100X Biotinylated FLRT2 Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 50 mL
  • Detector Antibody Diluent: 1 x 15 mL
  • Conjugate Diluent: 1 x 15 mL
  • 25X Wash Buffer: 1 x 20 mL
  • Stop Solution: 1 x 10 mL
  • TMB Substrate: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Urine, Tissue Homogenates
• Sensitivity: 23.44 pg/mL
• Assay Info: Assay Methodology: Quantitative Sanadwich ELISA

Target Info

• Gene Symbol: FLRT2
• Gene Full Name: fibronectin leucine rich transmembrane protein 2
• Alias Symbols: KIAA0405
• NCBI Gene Id: 23768
• Protein Name: Leucine-rich repeat transmembrane protein FLRT2
• Description of Target: Functions in cell-cell adhesion, cell migration and axon guidance. Mediates cell-cell adhesion via its interactions with ADGRL3 and probably also other latrophilins that are expressed at the surface of adjacent cells. May play a role in the migration of cortical neurons during brain development via its interaction with UNC5D. Mediates axon growth cone collapse and plays a repulsive role in neuron guidance via its interaction with UNC5D, and possibly also other UNC-5 family members. Plays a role in fibroblast growth factor-mediated signaling cascades. Required for normal organization of the cardiac basement membrane during embryogenesis, and for normal embryonic epicardium and heart morphogenesis.
• Uniprot ID: O43155
• Protein Accession #: NP_037363.1
• Nucleotide Accession #: NM_013231.5