FLOT1 Antibody (OAEB01735)

Catalog No: OAEB01735 (Formerly GWB-77859C)

Size:100UG

HEK293 overexpressing FLOT1(RC200231) and probed with OAEB01735 (mock transfection in first lane), tested by Origene.

(0.1ug/ml) staining of K562 lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

OAEB01735 staining (0.3ug/ml) of H460 lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

OAEB01735 (3.8ug/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

(5ug/ml) staining of paraffin embedded Human Kidney. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.

Negative Control showing staining of paraffin embedded Human Kidney, with no primary antibody.

Product Info

• Predicted Species Reactivity: Bovine|Dog|Human|Porcine
• Product Format: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Clonality: Polyclonal
• Host: Goat
• Application: IHC, WB
• Reconstitution and Storage: Aliquot and store at -20C. Minimize freezing and thawing.
• Immunogen: FLOT1
• Purification: Ammonium Sulfate Precipitated; Affinity Purified
• Predicted Homology Based on Immunogen Sequence: Cow: 100%; Dog: 100%; Horse: 100%; Human: 100%; Mouse: 92%; Pig: 100%; Rabbit: 100%; Rat: 92%
• Peptide Sequence: C-SISQVNHKPLRTA
• Concentration: 0.5 mg/ml
• Purity: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Application Info: Peptide ELISA: antibody detection limit dilution 1:32000.
  Western Blot: Approx 48kDa band observed in lysates of cell line H460 (calculated MW of 47.4kDa according to NP_005794.1). In transfected HEK293 transiently expressing FLOT1 a band of approx. 48kDa is observed. This band is not observed in the non-transfected HEK293. Recommended for use at 0.3-1ug/ml.
• Reference: * Vassilieva EV, Gerner-Smidt K, Ivanov AI, Nusrat A.; Lipid rafts mediate internalization of beta1-integrin in migrating intestinal epithelial cells.; Am J Physiol Gastrointest Liver Physiol. 2008 Nov;295(5):G965-76.; Pubmed 18755811
  * Masilamani M, Narayanan S, Prieto M, Borrego F, Coligan JE.; Uncommon endocytic and trafficking pathway of the natural killer cell CD94/NKG2A inhibitory receptor.; Traffic. 2008 Jun;9(6):1019-34.; Pubmed 18363778
  * Dolganiuc A, Bakis G, Kodys K, Mandrekar P, Szabo G.; Acute ethanol treatment modulates Toll-like receptor-4 association with lipid rafts. Alcohol Clin Exp Res. 2006 Jan;30(1):76-85.; Alcohol Clin Exp Res. 2006 Jan;30(1):76-85.; Pubmed 16433734
  * Bickel PE, Scherer PE, Schnitzer JE, Oh P, Lisanti MP, Lodish HF.; Flotillin and epidermal surface antigen define a new family of caveolae-associated integral membrane proteins.; J Biol Chem. 1997 May 23;272(21):13793-802.; Pubmed 9153235

Target Info

• Gene Symbol: Flot1|FLOT1
• Gene Full Name: flotillin 1
• Alias Symbols: flotillin-1;integral membrane component of caveolae;reggie;reggie-2.
• NCBI Gene Id: 10211|14251
• Protein Name: Flotillin-1
• Uniprot ID: O75955
• Protein Accession #: NP_005794.1
• Molecular Weight: 47.4; 42.1