FLIP Antibody (OAPB00027)

Catalog No: OAPB00027 (Formerly GWB-7AEEB5)

Size:100UG

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Western blot analysis of FLIP in NIH/3T3 whole cell lysate with FLIP antibody at 1:500 dilution.

Immunocytochemistry of FLIP in 3T3 cells with FLIP antibody at 5 μg/mL.

Product Info

• Predicted Species Reactivity: Human|Mouse
• Product Format: Liquid. FLIP Antibody is supplied in PBS containing 0.02% sodium azide.
• Clonality: Polyclonal
• Host: Rabbit
• Conjugation: Unconjugated
• Application: Enzyme-linked immunosorbent assay|Immunocytochemistry|Western blot
  Buffer: Antibody is supplied in PBS containing 0.02% sodium azide.
• Reconstitution and Storage: FLIP antibody can be stored at 4C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Immunogen: FLIP antibody was raised against a 17 amino acid peptide near the carboxy terminus of mouse FLIP. The immunogen is located within the last 50 amino acids of FLIP.
• Purification: Ion Exchange
• Predicted Homology Based on Immunogen Sequence: Mouse
• Concentration: 1 mg/ml
• Blocking Peptide: For anti-FLIP antibody is Catalog # OEPB02155
• Specificity: FLIP has short form (FLIPS) and long form (FLIPL) and antibody recognizes the FLIPL only.
• Application Info: FLIP antibody can be used for the detection of FLIP by Western blot at 1:500 to 1:1000 dilution. 55 kDa band can be detected. FLIP has short form (FLIPS) and long form (FLIPL) and antibody recognizes the FLIPL only. (Optimal dilution should be determined by user)
• Reference: Thome M, Schneider P, Hofmann K, Fickenscher H, Meinl E, Neipel F, Mattmann C, Burns K, Bodmer JL, Schroter M, Scaffidi C, Krammer PH, Peter ME, Tschopp J. Viral FLICE-inhibitory proteins (FLIPs) prevent apoptosis induced by death receptors. Nature 1997;386:517-521. ;Irmler M, Thome M, Hahne M, Schneider P, Hofmann K, Steiner V, Bodmer JL, Schroter M, Burns K, Mattmann C, Rimoldi D, French LE, Tschopp J. Inhibition of death receptor signals by cellular FLIP. Nature 1997;388:190-195. ;Shu HB, Halpin DR , Goeddel DV. Casper is a FADD- and caspase-related inducer of apoptosis. Immunity 1997;6:751-763.;Hu S, Vincenz C, Ni J, Gentz R, Dixit VM. I-FLICE, a novel inhibitor of tumor necrosis factor receptor-1- and CD-95-induced apoptosis. J Biol Chem 1997;272:17255-17257.

Target Info

• Gene Symbol: CFLAR
• Gene Full Name: CASP8 and FADD like apoptosis regulator
• Alias Symbols: CASH;CASP8 and FADD-like apoptosis regulator;CASP8AP1;caspase homolog;caspase-eight-related protein;caspase-like apoptosis regulatory protein;caspase-related inducer of apoptosis;Casper;cellular FLICE-like inhibitory protein;cFLIP;c-FLIP;c-FLIPL;c-FLIPR;c-FLIPS;CLARP;FADD-like antiapoptotic molecule 1;FLAME;FLAME1;FLAME-1;FLIP;I-FLICE;inhibitor of FLICE;MACH-related inducer of toxicity;MRIT;testis secretory sperm-binding protein Li 233m;Usurpin;usurpin beta.
• NCBI Gene Id: 8837
• Protein Name: CASP8 and FADD-like apoptosis regulator|FLICE-like inhibitory protein long form [Homo sapiens]
• Description of Target: Apoptosis regulator protein which may function as a crucial link between cell survival and cell death pathways in mammalian cells. Acts as an inhibitor of TNFRSF6 mediated apoptosis. A proteolytic fragment (p43) is likely retained in the death-inducing signaling complex (DISC) thereby blocking further recruitment and processing of caspase-8 at the complex. Full length and shorter isoforms have been shown either to induce apoptosis or to reduce TNFRSF-triggered apoptosis. Lacks enzymatic (caspase) activity.
• Uniprot ID: O15519
• Protein Accession #: https://www.ncbi.nlm.nih.gov/protein/AAC51622
• Molecular Weight: Predicted: 53 kDa Observed: 58 kDa