FKBP4 Antibody (OAGA01014)

Catalog No: OAGA01014

Size:100 ul

FKBP4 antibody detects FKBP4 protein by Western blot analysis.
A. 30 ug 293T whole cell lysate/extract
B. 30 ug A431 whole cell lysate/extract
C. 30 ug HeLa whole cell lysate/extract
D. 30 ug HepG2 whole cell lysate/extract
E. 30 ug A375 whole cell lysate/extract
10 % SDS-PAGE
FKBP4 antibody (OAGA01014) dilution: 1:1000

FKBP4 antibody detects FKBP4 protein by Western blot analysis.
A. 30 ug GL261 whole cell lysate/extract
B. 30 ug C8D30 whole cell lysate/extract
10 % SDS-PAGE
FKBP4 antibody (OAGA01014) dilution: 1:1000

FKBP4 antibody detects FKBP4 protein by Western blot analysis.
A. 30 ug Rat2 whole cell lysate/extract
10 % SDS-PAGE
FKBP4 antibody (OAGA01014) dilution: 1:1000

Immunofluorescence analysis of paraformaldehyde-fixed A431, using FKBP52(OAGA01014) antibody at 1:200 dilution.

Product Info

• Predicted Species Reactivity: Human, Mouse, Rat
• Product Format: Liquid
• Clonality: Polyclonal
• Isotype: IgG
• Host: Rabbit
• Application: ICC, IF, IHC-FFPE, WB
• Additional Information: Background: The protein encoded by this gene is a member of the immunophilin protein family, which play a role in immunoregulation and basic cellular processes involving protein folding and trafficking. This encoded protein is a cis-trans prolyl isomerase that binds to the immunosuppressants FK506 and rapamycin. It has high structural and functional similarity to FK506-binding protein 1A (FKBP1A), but unlike FKBP1A, this protein does not have immunosuppressant activity when complexed with FK506. It interacts with interferon regulatory factor-4 and plays an important role in immunoregulatory gene expression in B and T lymphocytes. This encoded protein is known to associate with phytanoyl-CoA alpha-hydroxylase. It can also associate with two heat shock proteins (hsp90 and hsp70) and thus may play a role in the intracellular trafficking of hetero-oligomeric forms of the steroid hormone receptors. This protein correlates strongly with adeno-associated virus type 2 vectors (AAV) resulting in a significant increase in AAV-mediated transgene expression in human cell lines. Thus this encoded protein is thought to have important implications for the optimal use of AAV vectors in human gene therapy. The human genome contains several non-transcribed pseudogenes similar to this gene. [provided by RefSeq]
  Positive Controls: 293T, A431, HeLa, HepG2, A375, GL261, C8D30, Rat2
  Cellular Localization: Cytoplasm, Nucleus
• Reconstitution and Storage: Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
• Immunogen: Recombinant fragment corresponding to a region within amino acids 1 and 207 of FKBP52 (Uniprot ID#Q02790)
• Purification: Purified by antigen-affinity chromatography.
• Concentration: 0.99 mg/ml
• Application Info: Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)], ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
• Storage Buffer: 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Target Info

• Gene Symbol: FKBP4
• Gene Full Name: Peptidyl-prolyl cis-trans isomerase FKBP4
• Alias Symbols: HBI, p52, Hsp56, FKBP51, FKBP52, FKBP59, PPIase
• NCBI Gene Id: 2288
• Protein Name: Peptidyl-prolyl cis-trans isomerase FKBP4
• Uniprot ID: Q02790
• Protein Accession #: NP_002005.1
• Molecular Weight: 52 kDa