FKBP1B ELISA Kit (Human) (OKCD00438)

Catalog No: OKCD00438 (Formerly GWB-KBBVU2)

Size:96WELLS

Human FKBP1B ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2 Dilution	1:4 Dilution	1:8 Dilution	1:16 Dilution
  	80-93%	83-101%	88-101%	90-98%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to FK506 Binding Protein 1B (FKBP1B). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to FK506 Binding Protein 1B (FKBP1B). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain FK506 Binding Protein 1B (FKBP1B), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of FK506 Binding Protein 1B (FKBP1B) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Recovery:

  Matrix	Recovery Range (%)	Average (%)
  	80-103	86

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level FK506 Binding Protein 1B (FKBP1B) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level FK506 Binding Protein 1B (FKBP1B) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-FKBP1B Microplate: 96 Wells (12 x 8 Well Strips)
  • FKBP1B Lyophilized Standard: 2 x 10 ng
  • 100X Biotinylated FKBP1B Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Tissue homogenates, cell lysates and other biological fluids.
• Sensitivity: < 0.053 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of FK506 Binding Protein 1B (FKBP1B). No significant cross-reactivity or interference between FK506 Binding Protein 1B (FKBP1B) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: FKBP1B
• Gene Full Name: FKBP prolyl isomerase 1B
• Alias Symbols: 12.6 kDa FK506-binding protein;12.6 kDa FKBP;calstabin 2;FK506 binding protein 1B, 12.6 kDa;FK506-binding protein 12.6;FK506-binding protein 1B;FKBP12.6;FKBP-12.6;FKBP-1B;FKBP1L;h-FKBP-12;immunophilin FKBP12.6;OTK4;peptidyl-prolyl cis-trans isomerase FKBP1B;PKBP1L;PPIase;PPIase FKBP1B;rotamase.
• NCBI Gene Id: 2281
• Protein Name: Peptidyl-prolyl cis-trans isomerase FKBP1B
• Description of Target: Has the potential to contribute to the immunosuppressive and toxic effects of FK506 and rapamycin. PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides.
• Uniprot ID: P68106
• Protein Accession #: NP_004107
• Nucleotide Accession #: NM_004116.4
• Protein Size (# AA): 108