Ficolin-1 ELISA Kit (Human) (OKBB00565)

Catalog No: OKBB00565

Size:96 Wells

Human Ficolin-1 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Human
• Application: ELISA
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: 2.5 Hours
• ELISA Kit Principle: Aviva's human Ficolin-1 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Ficolin-1 has been precoated onto 96-well plates. Standards(NS0, A30-A326) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Ficolin-1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the human Ficolin-1 amount of sample captured in plate.
• ELISA Kit Range: 0.78 ng/ml - 50 ng/ml
• ELISA Kit Reproducibility:

  	Intra-Assay Precision			Inter-Assay Precision
  Sample	1	2	3	1	2	3
  n	16	16	16	24	24	24
  Mean (pg/mL)	4.83	13.2	25.1	5.24	12.9	25.9
  SD	0.188	0.581	1.431	0.393	0.89	1.89
  CV%	3.9	4.4	5.7	7.5	6.9	7.3

• ELISA Kit Component:
  • 96-Well plate Pre-coated with Anti-Human Ficolin-1 Antibody: 1 x (12 x 8 Well Strip)
  • Lyophilized Recombinant Human Ficolin-1 Standard: 2 x 50 ng/tube
  • 100X Biotinylated Anti-Human Ficolin-1 Antibody: 1 x 130 uL
  • 100X Avidin-Biotin-Peroxidase Complex (ABC): 1 x 130 uL
  • Sample Diluent Buffer: 1 x 30 mL
  • Antibody Diluent Buffer: 1 x 12 mL
  • ABC Diluent Buffer: 1 x 12 mL
  • TMB Color Developing Agent: 1 x 10 mL
  • TMB Stop Solution: 1 x 10 mL
  • 10X Wash Buffer: 1 x 30 mL
• Reconstitution and Storage: Store at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
• Sample Type: Cell culture supernatants, serum and plasma (heparin)
• Sensitivity: <20 pg/ml
• Specificity: Natural and recombinant human Ficolin-1
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay
• Immunogen: Expression system for standard: NS0; Immunogen sequence: A30-A326
• Description: For quantitative detection of human Ficolin-1 in cell culture supernatants, serum and plasma(heparin).

Target Info

• Gene Symbol: FCN1
• Gene Full Name: ficolin (collagen/fibrinogen domain containing) 1
• Alias Symbols: Collagen/fibrinogen domain containing protein 1, Collagen/fibrinogen domain-containing protein 1, Fcn1, FCN1_HUMAN, FCNM, ficolin (collagen/fibrinogen domain containing) 1, Ficolin 1, Ficolin A, Ficolin alpha, Ficolin-1, Ficolin-A, Ficolin-alpha, M ficolin, M-ficolin
• NCBI Gene Id: 2219
• Protein Name: Ficolin-1
• Description of Target: Ficolin-1, and also commonly termed M-ficolin is a protein that in humans is encoded by the FCN1 gene. It is mapped to 9q34.3. The ficolin family of proteins are characterized by the presence of a leader peptide, a short N-terminal segment, followed by a collagen-like region, and a C-terminal fibrinogen-like domain. Ficolins have a crucial role in defense against pneumococcal infection through the lectin complement pathway. Ficolin 1 is predominantly expressed in the peripheral blood leukocytes, and has been postulated to function as a plasma protein with elastin-binding activity.
• Uniprot ID: O00602
• Protein Accession #: NP_001994.2
• Nucleotide Accession #: NM_002003.3