Fibronectin ELISA Kit (Human) (OKBB00145)

Catalog No: OKBB00145 (Formerly GWB-ZZD001)

Size:96 Tests

Human FN1 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Human
• Application: ELISA
• ELISA Kit Detection Method: Colorimetric, OD 450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: Aviva's human Fibronectin ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A polyclonal antibody from rabbit specific for Fibronectin has been precoated onto 96-well plates. Standards(from plasma) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Fibronectin is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the human Fibronectin amount of sample captured in plate.
• ELISA Kit Range: 156 pg/ml - 10,000 pg/ml
• ELISA Kit Component: 1. Lyophilized recombinant human FASL standard: 10ng/tube×2.
  2. One 96-well plate precoated with anti-human FASL antibody.
  3. Sample diluent buffer: 30 ml
  4. Biotinylated anti-human FASL antibod1. Lyophilized human Fibronectin standard: 10ng/tube×2.
  2. One 96-well plate precoated with anti-human Fibronectin antibody.
  3. Sample diluent buffer: 30 ml
  4. Biotinylated anti-human Fibronectin antibody: 130ul, dilution 1:100.
  5. Antibody diluent buffer: 12ml.
  6. Avidin-Biotin-Peroxidase Complex (ABC): 130ul, dilution 1:100.
  7. ABC diluent buffer: 12ml.
  8. TMB color developing agent: 10ml.
  9. TMB stop solution: 10ml.
• Additional Information: Range: 156pg/ml-10ng/ml
  Principle: Aviva's human Fibronectin ELISA Kit was based on standard sandwich
  enzyme-linked immune-sorbent assay technology. Human Fibronectin
  specific-specific polyclonal antibodies were precoated onto 96-well plates. The
  human specific detection polyclonal antibodies were biotinylated. The test
  samples and biotinylated detection antibodies were added to the wells
  subsequently and then followed by washing with PBS or TBS buffer.
  Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were
  washed away with PBS or TBS buffer. HRP substrate TMB was used to
  visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a
  blue color product that changed into yellow after adding acidic stop solution.
  The density of yellow is proportional to the human Fibronectin amount of
  sample captured in plate.
  Notes: 1. To inspect the validity of experiment operation and the appropriateness of sample dilution proportion, pilot
  experiment using standards and a small number of samples is recommended.
  2. The TMB Color Developing agent is colorless and transparent before using, contact us freely if it is not the case.
  3. Before using the Kit, spin tubes and bring down all components to the bottom of tubes.
  4. Duplicate well assay is recommended for both standard and sample testing.
  5. Don't let 96-well plate dry, for dry plate will inactivate active components on plate.
  6. Don't reuse tips and tubes to avoid cross contamination.
  7. To avoid to use the reagents from different batches together.
  8. In order to avoid marginal effect of plate incubation due to temperature difference ( reaction may be stronger in the
  marginal wells), it is suggested that the diluted ABC and TMB solution will be pre-warmed in 37C for 30 min before
  using.
  Background: Fibronectin (FN) also known as LETS, is identified on the surfFN of fibroblasts by labeling with radioactive compounds or specific antibodies. Fibronectin is a 430,000-dalton dimeric glycoprotein that exists in 2 forms, termed cellular and plasma fibronectin. Cellular and plasma fibronectins are heterodimers consisting of similar but not identical polypeptides.1 These two forms of FN differ in biologic activity. Fibronectins bind cell surfFNs and various compounds including collagen, fibrin, heparin, DNA, and actin. Because fibronectin stimulates endocytosis in several systems and promotes the clearance of particulate material from the circulation, it could function in the clearance of C1q-coated material such as immune complexes or cellular debris.2 Fibronectins are involved in cell adhesion, cell motility, opsonization, would healing, and maintenance of cell shape. LETS, encoded on chromosome 8, is responsible for the LETS protein expression in humans. Because LETS has been implicated in tumorigenicity and cellular transformation, it is of interest that rearrangement or modifications in the number of chromosome 8 have been associated with certain forms of cancer.3 The standard used in this kit is isolated from rat plasma with the molecular mass of 200-250KDa.
• Reconstitution and Storage: Store at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
• Sample Type: cell culture supernates, serum and plasma(heparin, EDTA, citrate)
• Sensitivity: <10 pg/ml
• Predicted Homology Based on Immunogen Sequence: No detectable cross-reactivity with any other cytokine.
• Reference: 1. Kornblihtt, A. R.; Umezawa, K.; Vibe-Pedersen, K.; Baralle, F. E. Primary structure of human fibronectin: differential
  splicing may generate at least 10 polypeptides from a single gene. EMBO J. 4: 1755-1759, 1985.
  2. Bing, D. H.; Almeda, S.; Isliker, H.; Lahav, J.; Hynes, R. O. Fibronectin binds to the C1q component of complement.
  Proc. Nat. Acad. Sci. 79: 4198-4201, 1982.
  3. Owerbach, D.; Doyle, D.; Shows, T. B. Genetics of the large, external, transformation-sensitive (LETS) protein:
  assignment of a gene coding for expression of LETS to human chromosome 8. Proc. Nat. Acad. Sci. 75: 5640-5644,
  1978.
• Specificity: Natural human Fibronectin
• Immunogen: Expression system for standard: from plasma
• Description: For quantitative detection of human Fibronectin in cell culture supernatants, serum and plasma(heparin, EDTA, citrate).

Target Info

• Gene Symbol: FN1
• Gene Full Name: fibronectin 1
• Alias Symbols: CIG, Cold insoluble globulin, Cold-insoluble globulin, Fibronectin 1, FINC, FINC_HUMAN, FN, FN1, FNZ, Migration stimulating factor, MSF, Ugl-Y3
• NCBI Gene Id: 2335
• Protein Name: Fibronectin
• Description of Target: Fibronectin (FN) also known as LETS, is identified on the surfFN of fibroblasts by labeling with radioactive compounds or specific antibodies. Fibronectin is a 430,000-dalton dimeric glycoprotein that exists in 2 forms, termed cellular and plasma fibronectin. Cellular and plasma fibronectins are heterodimers consisting of similar but not identical polypeptides.1 These two forms of FN differ in biologic activity. Fibronectins bind cell surfFNs and various compounds including collagen, fibrin, heparin, DNA, and actin. Because fibronectin stimulates endocytosis in several systems and promotes the clearance of particulate material from the circulation, it could function in the clearance of C1q-coated material such as immune complexes or cellular debris.2 Fibronectins are involved in cell adhesion, cell motility, opsonization, would healing, and maintenance of cell shape. LETS, encoded on chromosome 8, is responsible for the LETS protein expression in humans. Because LETS has been implicated in tumorigenicity and cellular transformation, it is of interest that rearrangement or modifications in the number of chromosome 8 have been associated with certain forms of cancer.3 The standard used in this kit is isolated from human plasma with the molecular mass of 200-250KDa.
• Uniprot ID: P02751
• Protein Accession #: NP_001293058.1
• Nucleotide Accession #: NM_001306129.1