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Catalog No: OKEH03684
Size:96T
Price: $625.00
SKU
OKEH03684
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityMouse, Mus musculus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology FHL2 ELISA Kit (Mouse) (OKEH03684) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Fhl2 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and removed. A biotinylated detector antibody specific for Fhl2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Fhl2 captured in well.
ELISA Kit Range78-5000pg/ml
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 96%
ELISA Kit ReproducibilityMean Intra-assay CV%: <= 6.6% (n = 20)
Mean Inter-assay CV%: <= 8.8% (n = 20)
ELISA Kit Component
ComponentAmount
Fhl2 Microplate96 Wells (12 x 8 Well strips)
Fhl2 Lyophilized Standard2
Sample Diluent1 x 20 mL
100X Biotinylated Fhl2 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Detector Antibody Diluent1 x 10 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Sensitivity39.09 pg/mL
SpecificityNatural and recombinant Mouse Four and a half LIM domains protein 2
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolFhl2
Gene Full Namefour and a half LIM domains 2
Alias SymbolsFHL-2;four and a half LIM domains protein 2;skeletal muscle LIM-protein 3;SL;SLIM3;SLIM-3.
NCBI Gene Id14200
Protein NameO70433
Description of TargetFour and a half LIM domains protein 2
Uniprot IDhttps://www.uniprot.org/uniprot/O70433
Protein Accession #NP_001276462.1
Nucleotide Accession #NM_001289533.1
Protein Size (# AA)279
Molecular Weight32 kDa
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