Catalog No: OKDD00265 (Formerly GWB-KBBTP6)
Size:96 Wells
Price: $650.00
SKU
OKDD00265
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Datasheets/ManualsPrintable datasheet for FGFR3 ELISA Kit (Human) (OKDD00265)
Product Info
Predicted Species ReactivityHomo sapiens|Human
ApplicationEnzyme-linked immunosorbent assay
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~4 Hours
ELISA Kit PrincipleAviva Systems Biology FGFR3 ELISA Kit (Human) (OKDD00265) is based on standard sandwich enzyme-linked immuno-sorbent assay methodology. An antibody for FGFR3 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for FGFR3 is added, incubated and removed. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample FGFR3 captured in well.
ELISA Kit Range0.156-10ng/mL
ELISA Kit Reproducibility

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level FGFR3 were tested 20 times on one plate, respectively.

Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level FGFR3 were tested on 3 different plates, 8 replicates in each plate.

Intra-Assay: CV<10%

Inter-Assay: CV<12%

ELISA Kit Component
ComponentAmount
Anti-FGFR3 Microplate96 Wells (12 x 8 Well Strips)
FGFR3 Lyophlized Standard2 x 10 ng
Standard Diluent1 x 20 mL
100X FGFR3 Biotinylated Detector Antibody1 x 120 uL
100X Avidin-HRP Detection Conjugate1 x 120 uL
Detector Antibody Diluent1 x 12 mL
Detection Conjugate Diluent1 x 12 mL
10X Sample Diluent1 x 30 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue homogenates, Cell lysates
Sensitivity< 0.055 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of FGFR3. No significant cross-reactivity or interference between FGFR3 and analogues was observed.
Assay InfoQuantitative Sandwich ELISA
Gene SymbolFGFR3
Gene Full Namefibroblast growth factor receptor 3
Alias SymbolsACH;CD333;CEK2;FGFR-3;fibroblast growth factor receptor 3;fibroblast growth factor receptor 3 variant 4;fibroblast growth factor receptor 3-S;HSFGFR3EX;hydroxyaryl-protein kinase;JTK4;tyrosine kinase JTK4.
NCBI Gene Id2261
Protein NameFibroblast growth factor receptor 3
Description of TargetTyrosine-protein kinase that acts as cell-surface receptor for fibroblast growth factors and plays an essential role in the regulation of cell proliferation, differentiation and apoptosis. Plays an essential role in the regulation of chondrocyte differentiation, proliferation and apoptosis, and is required for normal skeleton development. Regulates both osteogenesis and postnatal bone mineralization by osteoblasts. Promotes apoptosis in chondrocytes, but can also promote cancer cell proliferation. Required for normal development of the inner ear. Phosphorylates PLCG1, CBL and FRS2. Ligand binding leads to the activation of several signaling cascades. Activation of PLCG1 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate. Phosphorylation of FRS2 triggers recruitment of GRB2, GAB1, PIK3R1 and SOS1, and mediates activation of RAS, MAPK1/ERK2, MAPK3/ERK1 and the MAP kinase signaling pathway, as well as of the AKT1 signaling pathway. Plays a role in the regulation of vitamin D metabolism. Mutations that lead to constitutive kinase activation or impair normal FGFR3 maturation, internalization and degradation lead to aberrant signaling. Over-expressed or constitutively activated FGFR3 promotes activation of PTPN11/SHP2, STAT1, STAT5A and STAT5B. Secreted isoform 3 retains its capacity to bind FGF1 and FGF2 and hence may interfere with FGF signaling.
Uniprot IDP22607
Protein Accession #NP_000133.1
Nucleotide Accession #NM_000142.4
Protein Size (# AA)806
Molecular Weight88 kDa
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