Catalog No: OKEH04121
Size:96T
Price: $525.00
SKU
OKEH04121
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityRat, Rattus norvegicus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology Fgf9 ELISA Kit (Rat) (OKEH04121) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Fgf9 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Fgf9 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Fgf9 captured in the well.
ELISA Kit Range78-5000pg/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 105%
ELISA Kit ReproducibilityMean Intra-assay CV%: <= 5.5% (n = 20)
Mean Inter-assay CV%: <= 7.0% (n = 20)
ELISA Kit Component
ComponentAmount
Anti-Fgf9 Microplate96 Wells (12 x 8 Well Strips)
Fgf9 Lyophilized Standard2 x 2 ng
Sample Diluent1 x 20 mL
Fgf9 Biotinylated Detector Antibody1 x 120 uL
Detector Antibody Diluent1 x 12 mL
HRP Avidin Conjugate1 x 120 uL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
Sensitivity39.6 pg/mL
SpecificityNatural and recombinant Rat Fibroblast growth factor 9
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolFgf9
Gene Full Namefibroblast growth factor 9
Alias SymbolsFgf4b;FGF-9;fibroblast growth factor 4b;fibroblast growth factor 9;GAF;glia-activating factor;HBGF-9.
NCBI Gene Id25444
Protein NameP36364
Description of TargetFibroblast growth factor 9
Uniprot IDhttps://www.uniprot.org/uniprot/P36364
Protein Accession #NP_037084.1
Nucleotide Accession #NM_012952.1
Protein Size (# AA)208
Molecular Weight23 kDa
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