FGF7 ELISA Kit (Rat) (OKEH03008)

Catalog No: OKEH03008

Size:96T

Rat FGF7 ELISA kit standard curve

Product Info

• Predicted Species Reactivity: Rat, Rattus norvegicus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: Aviva Systems Biology FGF7 ELISA Kit (Rat) (OKEH03008) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Fgf7 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and removed. A biotinylated detector antibody specific for Fgf7 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Fgf7 captured in well.
• ELISA Kit Range: 15.6-1000pg/mL
• ELISA Kit Component:
  • Anti-Fgf7 Microplate: 96 Wells (12 x 8 Well strips)
  • Fgf7 Lyophilized Standard: 2
  • 100X Biotinylated Fgf7 Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
• Sensitivity: 7.88 pg/mL
• Specificity: Natural and recombinant Rat Fibroblast growth factor 7
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: Fgf7
• Gene Full Name: fibroblast growth factor 7
• Alias Symbols: Fgf5b;FGF-7;fibroblast growth factor 5b;fibroblast growth factor 7;HBGF-7;heparin-binding growth factor 7;Keratinocyte growth factor;Keratinocyte Growth Factor Fgf-7;Kgf;KGF/FGF7.
• NCBI Gene Id: 29348
• Protein Name: Q02195
• Description of Target: Fibroblast growth factor 7
• Uniprot ID: https://www.uniprot.org/uniprot/Q02195
• Protein Accession #: NP_071518.1
• Nucleotide Accession #: NM_022182.1
• Protein Size (# AA): 194
• Molecular Weight: 22 kDa