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Catalog No: OKEH00422
Size:96T
Price: $525.00
SKU
OKEH00422
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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FGF5 ELISA Kit (Human) : 96 Wells (OKEH00422)

Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Please click here to view the MSDS.
COA DatasheetPrintable COA datasheet for OKEH00422
Product Info
Predicted Species ReactivityHomo sapiens, Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology FGF5 ELISA Kit (Human) (OKEH00422) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Human FGF5 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. After washing,a biotinylated detector antibody specific for Human FGF5 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Human FGF5 captured in well.
ELISA Kit Range78-5000pg/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 98%
ELISA Kit ReproducibilityMean Intra-assay CV%: < 4.2% (n = 20)
Mean Inter-assay CV%: < 8.3% (n = 20)
ELISA Kit Component
ComponentAmount
Anti-FGF5 Microplate96 Wells (12 x 8 Well strips)
FGF5 Lyophilized Standard2
Sample Diluent1 x 20 mL
100X Biotinylated FGF51 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Sensitivity50 pg/mL
SpecificityNatural and recombinant Human Fibroblast growth factor 5
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolFGF5
Gene Full Namefibroblast growth factor 5
Alias Symbolsfibroblast growth factor 5;HBGF-5;heparin-binding growth factor 5;Smag-82;TCMGLY.
NCBI Gene Id2250
Protein NameP12034
Description of TargetFibroblast growth factor 5
Uniprot IDhttps://www.uniprot.org/uniprot/P12034
Protein Accession #NP_004455.2
Nucleotide Accession #NM_004464.3
Protein Size (# AA)268
Molecular Weight29 kDa
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