Catalog No: OKEH00284
Size:96T
Price: $425.00
SKU
OKEH00284
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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FGF21 ELISA Kit (Mouse) : 96 Wells (OKEH00284)

Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Please click here to view the MSDS.
Product Info
Predicted Species ReactivityMouse, Mus musculus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology FGF21 ELISA Kit (Mouse) (OKEH00284) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for FGF21 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. After washing,a biotinylated detector antibody specific for FGF21 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample FGF21 captured in well.
ELISA Kit Range15.6-1000pg/mL
ELISA Kit ReproducibilityMean Intra-assay CV%: < 4.2% (n = 20)
Mean Inter-assay CV%: < 6.9% (n = 20)
ELISA Kit Component
ComponentAmount
FGF21 Microplate96 Wells (12 x 8 Well strips)
FGF21 Lyophilized Standard2
Sample Diluent1 x 20 mL
100X Biotinylated FGF21 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Sensitivity7.8 pg/mL
SpecificityNatural and recombinant Mouse Fibroblast growth factor 21
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolFgf21
Gene Full Namefibroblast growth factor 21
Alias SymbolsFgf8c;fibroblast growth factor 21;fibroblast growth factor 8c.
NCBI Gene Id56636
Protein NameQ9JJN1
Description of TargetFibroblast growth factor 21
Uniprot IDhttps://www.uniprot.org/uniprot/Q9JJN1
Protein Accession #NP_064397.1
Nucleotide Accession #NM_020013.4
Protein Size (# AA)210
Molecular Weight23 kDa
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