FGF2 ELISA Kit (Chicken) (OKCD02547)

Catalog No: OKCD02547

Size:96WELLS

Chicken FGF2 ELISA kit standard curve

Product Info

• Predicted Species Reactivity: Chicken|Gallus gallus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2	1:4	1:8	1:16
  Serum (n=5)	87-104%	95-105%	91-101%	97-105%
  EDTA Plasma (n=5)	82-91%	84-98%	80-93%	90-97%
  Heparin Plasma (n=5)	78-92%	83-104%	98-105%	96-104%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Fibroblast Growth Factor 2, Basic (FGF2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Fibroblast Growth Factor 2, Basic (FGF2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Fibroblast Growth Factor 2, Basic (FGF2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Fibroblast Growth Factor 2, Basic (FGF2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 15.6-1,000pg/mL
• ELISA Kit Recovery:

  Matrix	Recovery range (%)	Average(%)
  Serum (n=5)	82-93	86
  EDTA Plasma (n=5)	83-101	94
  Heparin Plasma(n=5)	78-96	84

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Fibroblast Growth Factor 2, Basic (FGF2) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Fibroblast Growth Factor 2, Basic (FGF2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-FGF2 Microplate: 96 Wells (12 x 8 Well strips)
  • FGF2 Lyophilized Standard: 2
  • 100X Biotinylated FGF2 Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • Stop Solution: 1 x 6 mL
  • TMB Substrate: 1 x 9 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma and other biological fluids
• Sensitivity: 6.3 pg/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Fibroblast Growth Factor 2, Basic (FGF2). No significant cross-reactivity or interference between Fibroblast Growth Factor 2, Basic (FGF2) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: FGF2
• Gene Full Name: fibroblast growth factor 2
• Alias Symbols: basic fibroblast growth factor;BFGF;FGF-2;fibroblast growth factor 2;fibroblast growth factor 2 (basic);HBGF-2;heparin-binding growth factor 2.
• NCBI Gene Id: 396413
• Protein Name: Fibroblast growth factor 2
• Description of Target: Acts as a ligand for FGFR1, FGFR2, FGFR3 and FGFR4. Also acts as an integrin ligand which is required for FGF2 signaling. Plays an important role in the regulation of cell survival, cell division, cell differentiation and cell migration. Functions as a potent mitogen in vitro. Can induce angiogenesis.
• Uniprot ID: P48800
• Protein Accession #: NP_990764.1
• Nucleotide Accession #: NM_205433.1
• Protein Size (# AA): 158