FGF2 ELISA Kit (Cattle) (OKCD02548)

Catalog No: OKCD02548

Size:96WELLS

FGF2 ELISA kit standard curve

Product Info

• Predicted Species Reactivity: Bos taurus|Bovine
• Application: Enzyme-linked Immunosorbent assay-Competitive
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 2h
• ELISA Kit Linearity:

  Sample	1:2	1:4	1:8	1:16
  Serum (n=5)	90-99%	91-101%	97-104%	87-94%
  EDTA Plasma (n=5)	86-101%	91-102%	78-94%	81-96%
  Heparin Plasma (n=5)	95-104%	79-102%	84-98%	91-98%

• ELISA Kit Principle: This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Fibroblast Growth Factor 2, Basic (FGF2) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Fibroblast Growth Factor 2, Basic (FGF2) and unlabeled Fibroblast Growth Factor 2, Basic (FGF2) (Standards or samples) with the pre-coated antibody specific to Fibroblast Growth Factor 2, Basic (FGF2). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Fibroblast Growth Factor 2, Basic (FGF2) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Fibroblast Growth Factor 2, Basic (FGF2) in the sample.
• ELISA Kit Range: 12.35-1,000pg/mL
• ELISA Kit Recovery:

  Matrix	Recovery range (%)	Average(%)
  Serum (n=5)	89-101	95
  EDTA Plasma (n=5)	86-95	91
  Heparin Plasma (n=5)	79-99	81

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Fibroblast Growth Factor 2, Basic (FGF2) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Fibroblast Growth Factor 2, Basic (FGF2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
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• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Sensitivity: < 4.49 pg/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Fibroblast Growth Factor 2, Basic (FGF2). No significant cross-reactivity or interference between Fibroblast Growth Factor 2, Basic (FGF2) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Competitive Inhibition ELISA

Target Info

• Gene Symbol: FGF2
• Gene Full Name: Fibroblast growth factor 2
• Alias Symbols: Basic fibroblast growth factor;Heparin-binding growth factor 2.
• NCBI Gene Id: 281161
• Protein Name: Fibroblast growth factor 2
• Description of Target: Acts as a ligand for FGFR1, FGFR2, FGFR3 and FGFR4. Also acts as an integrin ligand which is required for FGF2 signaling. Binds to integrin ITGAV:ITGB3. Plays an important role in the regulation of cell survival, cell division, cell differentiation and cell migration. Functions as a potent mitogen in vitro. Can induce angiogenesis.
• Uniprot ID: P03969
• Protein Accession #: NP_776481.2
• Nucleotide Accession #: NM_174056.4
• Molecular Weight: 155 kDa