FGF13 ELISA Kit (Rat) (OKCD00187)

Catalog No: OKCD00187 (Formerly GWB-KBBSC9)

Size:96WELLS

Rat Fgf13 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Rat|Rattus norvegicus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2 Dilution	1:4 Dilution	1:8 Dilution	1:16 Dilution
  serum(n=5)	88-101%	92-105%	89-97%	88-99%
  EDTA plasma(n=5)	78-91%	78-88%	84-93%	98-105%
  heparin plasma(n=5)	78-95%	97-105%	78-90%	94-103%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Fibroblast Growth Factor 13 (FGF13). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Fibroblast Growth Factor 13 (FGF13). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Fibroblast Growth Factor 13 (FGF13), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Fibroblast Growth Factor 13 (FGF13) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 15.62-1000pg/mL
• ELISA Kit Recovery:

  Matrix	Recovery Range (%)	Average (%)
  serum(n=5)	88-96	92
  EDTA plasma(n=5)	89-103	99
  heparin plasma(n=5)	84-98	89

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Fibroblast Growth Factor 13 (FGF13) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Fibroblast Growth Factor 13 (FGF13) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-Fgf13 Microplate: 96 Wells (12 x 8 Well Strips)
  • Fgf13 Lyophilized Standard: 2 x 1 ng
  • 100X Biotinylated Fgf13 Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma and other biological fluids.
• Sensitivity: < 5.4 pg/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Fibroblast Growth Factor 13 (FGF13). No significant cross-reactivity or interference between Fibroblast Growth Factor 13 (FGF13) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: Fgf13
• Gene Full Name: fibroblast growth factor 13
• Alias Symbols: FGF-13;Fgf1c;fibroblast growth factor 13;fibroblast growth factor 1c.
• NCBI Gene Id: 84488
• Protein Name: Fibroblast growth factor 13
• Description of Target: Microtubule-binding protein which directly binds tubulin and is involved in both polymerization and stabilization of microtubules. Through its action on microtubules, may participate to the refinement of axons by negatively regulating axonal and leading processes branching. Plays a crucial role in neuron polarization and migration in the cerebral cortex and the hippocampus (By similarity).
• Uniprot ID: Q9ERW3
• Protein Accession #: NP_445880
• Nucleotide Accession #: NM_053428.1
• Protein Size (# AA): 245