FEN1 Antibody (OAAF02255)

Catalog No: OAAF02255 (Formerly GWB-ASD503)

Size:100 ug

Western blot analysis of extracts from COLO205 cells, using FEN1 Antibody. The lane on the right is treated with the synthesized peptide.

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using FEN1 Antibody. The picture on the right is treated with the synthesized peptide.

Immunofluorescence analysis of HeLa cells, using FEN1 Antibody. The picture on the right is treated with the synthesized peptide.

Product Info

• Predicted Species Reactivity: Human|Mouse|Rat
• Product Format: Liquid
• Clonality: Polyclonal
• Host: Rabbit
• Application: Enzyme-linked immunosorbent assay|Immunocytochemistry|Immunofluorescence|Immunohistochemistry|Immunohistochemistry-Paraffin|Western blot
• Reconstitution and Storage: -20°C
• Immunogen: The antiserum was produced against synthesized peptide derived from human FEN1.
• Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Specificity: FEN1 antibody detects endogenous levels of total FEN1 protein.
• Formulation: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Dilution: The optimal working dilution should be determined by the end user.
• Application Info: WB: 1:500~1:3000; IHC: 1:50~1:100; IF: 1:100~1:500; ELISA: 1:5000
• Reference: Murray J.M., Mol. Cell. Biol. 14:4878-4888(1994).
  Hiraoka L.R., Genomics 25:220-225(1995).
  Taylor T.D., Nature 440:497-500(2006).

Target Info

• Gene Symbol: FEN1
• Gene Full Name: flap structure-specific endonuclease 1
• Alias Symbols: DNase IV;FEN-1;flap endonuclease 1;Flap structure-specific endonuclease 1;Maturation factor 1;maturation factor-1;MF1;RAD2.
• NCBI Gene Id: 2237
• Protein Name: Flap endonuclease 1
• Description of Target: Structure-specific nuclease with 5'-flap endonuclease and 5'-3' exonuclease activities involved in DNA replication and repair. During DNA replication, cleaves the 5'-overhanging flap structure that is generated by displacement synthesis when DNA polymerase encounters the 5'-end of a downstream Okazaki fragment. It enters the flap from the 5'-end and then tracks to cleave the flap base, leaving a nick for ligation. Also involved in the long patch base excision repair (LP-BER) pathway, by cleaving within the apurinic/apyrimidinic (AP) site-terminated flap. Acts as a genome stabilization factor that prevents flaps from equilibrating into structurs that lead to duplications and deletions. Also possesses 5'-3' exonuclease activity on nicked or gapped double-stranded DNA, and exhibits RNase H activity. Also involved in replication and repair of rDNA and in repairing mitochondrial DNA.
• Uniprot ID: P39748
• Protein Accession #: NP_004102.1
• Molecular Weight: 42 kDa