Catalog No: OKEH04594
Size:96T
Price: $525.00
SKU
OKEH04594
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHomo sapiens, Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~3 Hours
ELISA Kit PrincipleAviva Systems Biology FCN3 ELISA Kit (Human) (OKEH04594) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for FCN3 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for FCN3 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample FCN3 captured in well.
ELISA Kit Range0.78-50ng/mL
ELISA Kit ReproducibilityMean Intra-Assay CV: < 10%
Mean Inter-Assay CV: < 12%
ELISA Kit Component
ComponentAmount
Anti-FCN3 Microplate96 Wells (12 x 8 Well Strips)
FCN3 Lyophilized Standard2
Sample Diluent1 x 20 mL
FCN3 Biotinylated Detector Antibody1 x 120 uL
Detector Antibody Diluent1 x 12 mL
HRP Avidin Conjugate1 x 120 uL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Sensitivity0.34 ng/mL
SpecificityNatural and recombinant Human Ficolin-3
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolFCN3
Gene Full Nameficolin 3
Alias Symbolscollagen/fibrinogen domain-containing lectin 3 p35;collagen/fibrinogen domain-containing protein 3;FCNH;ficolin (collagen/fibrinogen domain containing) 3 (Hakata antigen);ficolin-3;HAKA1;Hakata antigen;H-ficolin.
NCBI Gene Id8547
Protein NameO75636
Description of TargetFicolin-3
Uniprot IDhttps://www.uniprot.org/uniprot/O75636
Protein Accession #NP_003656.2
Nucleotide Accession #NM_003665.2
Protein Size (# AA)299
Molecular Weight33 KDa
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