Catalog No: OKEH02039
Size:96T
Price: $600.00
SKU
OKEH02039
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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FCGR3B ELISA Kit (Human) : 96 Wells (OKEH02039)

Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
COA DatasheetPrintable COA datasheet for OKEH02039
Product Info
Predicted Species ReactivityHomo sapiens, Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology FCGR3B ELISA Kit (Human) (OKEH02039) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for FCGR3B has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for FCGR3B is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample FCGR3B captured in well.
ELISA Kit Range0.312-20ng/mL
ELISA Kit Recovery<= 4.9%
ELISA Kit ReproducibilityMean Intra-assay CV%: < 6.5% (n = 20)
Mean Inter-assay CV%: < 9.9% (n = 20)
ELISA Kit Component
ComponentAmount
FCGR3B Microplate96 Wells (12 x 8 Well strips)
FCGR3B Lyophilized Standard2
Sample Diluent1 x 20 mL
100X GPX3-Biotin Complex1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Sensitivity0.11 ng/mL
SpecificityNatural and recombinant Human Low affinity immunoglobulin gamma Fc region receptor III-B
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolFCGR3B
Gene Full NameFc fragment of IgG receptor IIIb
Alias SymbolsCD16;CD16A;CD16b;Fc fragment of IgG, low affinity IIIb, receptor (CD16b);Fc gamma receptor IIIb;Fc gamma receptor III-B;FCG3;Fc-gamma receptor IIIb (CD 16);fc-gamma RIIIb;fc-gamma RIII-beta;FCGR3;FCGR3A;FCR-10;FCRIII;FCRIIIb;igG Fc receptor III-1;low affinity immunoglobulin gamma Fc region receptor III-B.
NCBI Gene Id2215
Protein NameO75015
Description of TargetLow affinity immunoglobulin gamma Fc region receptor III-B
Uniprot IDhttps://www.uniprot.org/uniprot/O75015
Protein Accession #NP_000561.3
Nucleotide Accession #NM_000570.4
Protein Size (# AA)233
Molecular Weight26 kDa
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