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Catalog No: OKEH04247
Size:96T
Price: $525.00
SKU
OKEH04247
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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  • $55: Antibody & Protein in US
  • $55 + $25/Kit in US
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityMouse, Mus musculus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology Fcgr1 ELISA Kit (Mouse) (OKEH04247) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Fcgr1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Fcgr1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Fcgr1 captured in the well.
ELISA Kit Range31.2-2000pg/ml
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 101%
ELISA Kit ReproducibilityMean Intra-assay CV%: <= 6.2% (n = 20)
Mean Inter-assay CV%: <= 7.7% (n = 20)
ELISA Kit Component
ComponentAmount
Fcgr1 Microplate96 Wells (12 x 8 Well Strips)
Fcgr1 Standard2 x 2 ng
Sample Diluent1 x 20 mL
Fcgr1 Biotinylated Detector Antibody1 x 120 uL
Detector Antibody Diluent1 x 12 mL
HRP Avidin Conjugate1 x 120 uL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue homogenates, Cell culture supernates and Other biological fluids.
Sensitivity15.74 pg/mL
SpecificityNatural and recombinant Mouse High affinity immunoglobulin gamma Fc receptor I
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolFcgr1
Gene Full NameFc receptor, IgG, high affinity I
Alias SymbolsAI323638;AV092959;CD64;Fcgam;fc-gamma RI;FcgammaRI;fcRI;high affinity immunoglobulin gamma Fc receptor I;igG Fc receptor I;IgG high affinity Fc receptor;IGGHAFC.
NCBI Gene Id14129
Protein NameP26151
Description of TargetHigh affinity immunoglobulin gamma Fc receptor I
Uniprot IDhttps://www.uniprot.org/uniprot/P26151
Protein Accession #NP_034316.1
Nucleotide Accession #NM_010186.5
Protein Size (# AA)404
Molecular Weight45 kDa
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