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Catalog No: OKEH04108
Size:96T
Price: $475.00
SKU
OKEH04108
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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  • $55: Antibody & Protein in US
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHomo sapiens, Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology FCER2 ELISA Kit (Human) (OKEH04108) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for FCER2 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for FCER2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample FCER2 captured in the well.
ELISA Kit Range78-5000pg/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 94%
ELISA Kit ReproducibilityMean Intra-assay CV%: <= 4.2% (n = 20)
Mean Inter-assay CV%: <= 8.0% (n = 20)
ELISA Kit Component
ComponentAmount
Anti-FCER2 Microplate96 Wells (12 x 8 Well Strips)
FCER2 Lyophilized Standard2 x 2 ng
Sample Diluent1 x 20 mL
FCER2 Biotinylated Detector Antibody1 x 120 uL
Detector Antibody Diluent1 x 12 mL
HRP Avidin Conjugate1 x 120 uL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
Sensitivity34 pg/mL
SpecificityNatural and recombinant Human Low affinity immunoglobulin epsilon Fc receptor
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolFCER2
Gene Full NameFc fragment of IgE receptor II
Alias SymbolsBLAST-2;CD23;CD23 antigen;CD23A;CLEC4J;C-type lectin domain family 4 member J;C-type lectin domain family 4, member J;Fc epsilon receptor II;Fc fragment of IgE, low affinity II, receptor for (CD23);FCE2;fc-epsilon-RII;IGEBF;immunoglobulin E-binding factor;immunoglobulin epsilon-chain;low affinity immunoglobulin epsilon Fc receptor;lymphocyte IgE receptor.
NCBI Gene Id2208
Protein NameP06734
Description of TargetLow affinity immunoglobulin epsilon Fc receptor
Uniprot IDhttps://www.uniprot.org/uniprot/P06734
Protein Accession #NP_001193948.2
Nucleotide Accession #NM_001207019.2
Protein Size (# AA)321
Molecular Weight36 kDa
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