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Catalog No: OKCD00804 (Formerly GWB-KBBGZ2)
Size:96WELLS
Price: $650.00
SKU
OKCD00804
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Datasheets/ManualsPrintable datasheet for FBN1 ELISA Kit (Rat) (OKCD00804)
Product Info
Predicted Species ReactivityRat|Rattus norvegicus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit Linearity
Sample1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
serum(n=5)94-103%97-105%84-94%98-105%
EDTA plasma(n=5)94-101%80-97%98-105%89-97%
heparin plasma(n=5)88-101%79-93%84-95%99-105%
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Fibrillin 1 (FBN1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Fibrillin 1 (FBN1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Fibrillin 1 (FBN1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Fibrillin 1 (FBN1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.312-20ng/mL
ELISA Kit Recovery
MatrixRecovery Range (%)Average (%)
serum(n=5)82-10195
EDTA plasma(n=5)91-10296
heparin plasma(n=5)78-9186
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Fibrillin 1 (FBN1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Fibrillin 1 (FBN1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-Fbn1 Microplate96 Wells (12 x 8 Well Strips)
Fbn1 Lyophilized Standard2 x 20 ng
100X Biotinylated Fbn1 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSerum, plasma, tissue homogenates and other biological fluids.
Sensitivity< 0.107 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Fibrillin 1 (FBN1). No significant cross-reactivity or interference between Fibrillin 1 (FBN1) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
Gene SymbolFbn1
Gene Full Namefibrillin 1
Alias Symbolsfibrillin-1.
NCBI Gene Id83727
Protein NameFibrillin 1
Description of Target#N/A
Uniprot IDQ9WUH8
Protein Accession #NP_114013
Nucleotide Accession #NM_031825.1
Protein Size (# AA)2872
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