Fas Ligand ELISA Kit (Human) : 96 Wells (OKAG00119)

Catalog No: OKAG00119 (Formerly GWB-SKR119)

Size:12x8WELLS

Fas Ligand ELISA Kit (Human) (OKAG00119) Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens, Human
• Product Format: 1 x 96-Well Plate or 12 x 8-Well Strips
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric 450 nm
• ELISA Kit Duration: 4.5 hours
• ELISA Kit Principle: The Aviva Human Fas-Ligand ELISA Kit contains the components necessary for quantitative determination of natural or recombinant hFas-Ligand concentrations within any experimental sample including cell lysates, serum and plasma. This particular immunoassay utilizes the quantitative technique of a "Sandwich" Enzyme-Linked Immunosorbent Assay (ELISA) where the target protein (antigen) is bound in a "sandwich" format by the primary capture antibodies coated to each well-bottom and the secondary detection antibodies added subsequently by the investigator.The capture antibodies coated to the bottom of each well are specific for a particular epitope on theHuman Fas-Ligand cytokine while the user-added detection antibodies bind to epitopes on the captured target protein. Amid each step of the procedure, a series of wash steps must be performed to ensure the elimination of non-specific binding between proteins to other proteins or to the solid phase. After incubation and "sandwiching" of the target antigen, a peroxidase enzyme is conjugated to the constant heavy chain of the secondary antibody (either covalently or via Avidin/Streptavidin-Biotin interactions), allowing for a colorimetricreaction to ensue upon substrate addition. When the substrate TMB (3, 3’, 5, 5’-Tetramethylbenzidine) is added, the reaction catalyzed by peroxidase yields a blue color that is representative of the antigen concentration. Upon sufficient color development, the reaction can be terminated through addition of Stop Solution (2 N Sulfuric Acid) where the color of the solution will turn yellow. The absorbance of each well can then be read by a spectrophotometer, allowing for generation of a standard curve and subsequent determination of protein concentration.
• ELISA Kit Range: 32-2000 pg/ml
• ELISA Kit Component:
  • Capture Antibody Coated Microplate: 96 Wells (12 x 8)
  • Ready-to-Use Streptavidin-HRP: 12 mL
  • Ready-to-Use Substrate: 12 mL
  • Stop Solution: 12 mL
  • Wash Buffer (15x): 50 mL
  • Protein Standard Diluent: 12 mL
  • Sample Diluent: 12 mL
  • Detection Antibody Diluent: 12 mL
  • Biotin-Labeled Detection Antibody: Lyophilized
  • Protein Standard: Lyophilized (8 ng)
• Reconstitution and Storage: 2°C to 8°C
• Sample Type: Cell lysates, sera and plasma
• Sensitivity: 32 pg/mL
• Specificity: The Human Fas-Ligand ELISA is capable of recognizing both recombinant and naturally produced Human Fas-Ligand proteins. The antigens listed below were tested at 50 ng/ml and did not exhibit significant cross reactivity or interference.
  • Human: APRIL, Fas/Fc Chimera, GITR/Fc Chimera, LIGHT, OX40 Ligand, TNF-α, TRANCE, TWEAK
  • Murine: Fas/Fc Chimera, Fas Ligand
• Assay Info: Quantitative Colorimentric Sandwich ELISA

Target Info

• Gene Symbol: FASLG
• Gene Full Name: Fas ligand
• Alias Symbols: ALPS1B;apoptosis (APO-1) antigen ligand 1;apoptosis antigen ligand;APT1LG1;APTL;CD178;CD95 ligand;CD95L;CD95-L;fas antigen ligand;Fas ligand (TNF superfamily, member 6);FASL;mutant tumor necrosis factor family member 6;TNFSF6;TNLG1A;tumor necrosis factor ligand 1A;tumor necrosis factor ligand superfamily member 6.
• NCBI Gene Id: 356
• Protein Name: P48023
• Description of Target: Tumor necrosis factor ligand superfamily member 6
• Uniprot ID: https://www.uniprot.org/uniprot/P48023
• Protein Accession #: NP_000630.1
• Nucleotide Accession #: NM_000639.2