Catalog No: OKBB00494
Size:96 Wells
Price: $583.00
SKU
OKBB00494
Availability: Domestic: within 1-2 week delivery | International: within 1-2 week delivery
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  • $55: Antibody & Protein in US
  • $55 + $25/Kit in US
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
COA DatasheetPrintable COA datasheet for OKBB00494
Product Info
Predicted Species ReactivityRat
ApplicationELISA
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration2.5 Hours
ELISA Kit PrincipleAviva's rat FASL ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for FASL has been precoated onto 96-well plates. Standards(NS0, Q127-L278) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for FASL is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the rat FASL amount of sample captured in plate.
ELISA Kit Range31.2 pg/ml - 2,000 pg/ml
ELISA Kit Reproducibility
Intra-Assay PrecisionInter-Assay Precision
Sample123123
n 161616242424
Mean (pg/mL)11885413191028471423
SD4.1340.9971.235.252.51103.9
CV%3.54.85.45.16.27.3
ELISA Kit Component
ComponentAmount
96-Well plate Pre-coated with Anti-Rat FAS-L Antibody1 x (12 x 8 Well Strip)
Lyophilized Recombinant Rat FAS-L Standard2 x 10 ng/tube
100X Biotinylated Anti-Rat FAS-L Antibody1 x 130 uL
100X Avidin-Biotin-Peroxidase Complex (ABC)1 x 130 uL
Sample Diluent Buffer1 x 30 mL
Antibody Diluent Buffer1 x 12 mL
ABC Diluent Buffer1 x 12 mL
TMB Color Developing Agent1 x 10 mL
TMB Stop Solution1 x 10 mL
10X Wash Buffer1 x 30 mL
Reconstitution and StorageStore at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Sample TypeCell culture supernatants, serum and plasma (heparin, EDTA, citrate)
Sensitivity<10 pg/ml
SpecificityNatural and recombinant rat FAS-L
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
ImmunogenExpression system for standard: NS0; Immunogen sequence: Q127-L278
DescriptionFor quantitative detection of rat FAS-L in cell culture supernatants, serum and plasma(heparin, EDTA, citrate).
Gene SymbolFASLG
Gene Full NameFas ligand (TNF superfamily, member 6)
Alias SymbolsApoptosis (APO 1) antigen ligand 1, Apoptosis antigen ligand, APT1LG1, APTL, CD178, CD95 L, CD95 ligand, CD95L, CD95L protein, Fas antigen ligand, FASL, FASLG, TNF superfamily member 6, TNFSF6, Tumor necrosis factor (ligand) superfamily member 6, Tumor necrosis factor ligand superfamily member 6
NCBI Gene Id25385
Protein NameTumor necrosis factor ligand superfamily member 6
Description of TargetFAS Ligand (FASL) is a 40 kDa type II membrane protein belonging to the tumor necrosis factor family, which induces apoptosis by binding to its receptor, Fas. The human FasL gene consists of approximately 8.0 kb and is split into four exons. This gene consists of 281 amino acids with a calculated M (r) of 31,759 and was mapped on chromosome 1q23. It has an identity of 76.9% at the amino acid sequence level with mouse FasL. The FAS and FASL system plays a key role in regulating apoptotic cell death and corruption of this signalling pathway has been shown to participate in immune escape and tumorigenesis. FAS and FASL triggered apoptosis pathway plays an important role in human carcinogenesis. This system may also play a role in modulating the genetic susceptibility of mouse strains to develop T-cell lymphoblastic lymphomas.
Uniprot IDP36940
Protein Accession #NP_037040.1
Nucleotide Accession #NM_012908.1
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