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Catalog No: OKEH00048
Size:96T
Price: $425.00
SKU
OKEH00048
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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FAS ELISA Kit (Human) : 96 Wells (OKEH00048)

Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHomo sapiens, Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology FAS ELISA Kit (Human) (OKEH00048) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for FAS has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for FAS is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample FAS captured in well.
ELISA Kit Range78-5000pg/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 96%
ELISA Kit ReproducibilityMean Intra-assay CV%: < 3.6% (n = 20)
Mean Inter-assay CV%: < 6.8% (n = 20)
ELISA Kit Component
ComponentAmount
FAS Microplate96 Wells (12 x 8 Well strips)
FAS Lyophilized Standard2
100X Biotinylated FAS Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Sample Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
Sensitivity24 pg/mL
SpecificityNatural and recombinant Human Tumor necrosis factor receptor superfamily member 6
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolFAS
Gene Full NameFas cell surface death receptor
Alias SymbolsALPS1A;APO-1;Apo-1 antigen;APO-1 cell surface antigen;apoptosis antigen 1;apoptosis signaling receptor FAS;apoptosis-mediating surface antigen FAS;APT1;CD95;CD95 antigen;Fas (TNF receptor superfamily, member 6);Fas AMA;FAS1;FASLG receptor;FASTM;mutant tumor necrosis receptor superfamily member 6;TNF receptor superfamily member 6;TNFRSF6;tumor necrosis factor receptor superfamily member 6;tumor necrosis factor receptor superfamily, member 6.
NCBI Gene Id355
Protein NameP25445
Description of TargetTumor necrosis factor receptor superfamily member 6
Uniprot IDhttps://www.uniprot.org/uniprot/P25445
Protein Accession #NP_000034
Nucleotide Accession #NM_000043
Protein Size (# AA)335
Molecular Weight38 kDa
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