Catalog No: OKEH02368
Size:96T
Price: $625.00
SKU
OKEH02368
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHomo sapiens, Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology FAM3A ELISA Kit (Human) (OKEH02368) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for FAM3A has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for FAM3A is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample FAM3A captured in well.
ELISA Kit Range0.156-10ng/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 97%
ELISA Kit ReproducibilityMean Intra-assay CV%: < 6.1% (n = 20)
Mean Inter-assay CV%: < 9.2% (n = 20)
ELISA Kit Component
ComponentAmount
FAM3A Microplate96 Wells (12 x 8 Well strips)
FAM3A Lyophilized Standard2
Sample Diluent1 x 20 mL
100X Biotinylated FAM3A Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Sensitivity0.098 ng/mL
SpecificityNatural and recombinant Human Protein FAM3A
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolFAM3A
Gene Full NameFAM3 metabolism regulating signaling molecule A
Alias Symbols2.19;cytokine-like protein 2-19;DLD;DXS560S;family with sequence similarity 3 member A;protein FAM3A;XAP-7.
NCBI Gene Id60343
Protein NameP98173
Description of TargetProtein FAM3A
Uniprot IDhttps://www.uniprot.org/uniprot/P98173
Protein Accession #NP_001164603.1
Nucleotide Accession #NM_001171132.2
Protein Size (# AA)230
Molecular Weight25 kDa
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