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Catalog No: OKEH03200
Size:96T
Price: $525.00
SKU
OKEH03200
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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  • $55: Antibody & Protein in US
  • $55 + $25/Kit in US
  • Contact us for international orders.
Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
COA DatasheetPrintable COA datasheet for OKEH03200
Product Info
Predicted Species ReactivityRat, Rattus norvegicus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology F2 ELISA Kit (Rat) (OKEH03200) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for F2 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and removed. A biotinylated detector antibody specific for F2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample F2 captured in well.
ELISA Kit Range1.56-100ng/mL
ELISA Kit Component
ComponentAmount
Anti-F2 Microplate96 Wells (12 x 8 Well strips)
F2 Lyophilized Standard2
100X Biotinylated F2 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Sample Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
Sensitivity0.81 ng/mL
SpecificityNatural and recombinant Rat Prothrombin
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolF2
Gene Full Namecoagulation factor II
Alias SymbolsCoagulation factor II.
NCBI Gene Id29251
Protein NameP18292
Description of TargetProthrombin
Uniprot IDhttps://www.uniprot.org/uniprot/P18292
Protein Accession #NP_075213.2
Nucleotide Accession #NM_022924.2 
Protein Size (# AA)617
Molecular Weight70 kDa
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