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Datasheets/Manuals | Printable datasheet for EZR Antibody (Phospho-Tyr566) (OAAF00316) |
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Predicted Species Reactivity | Human|Mouse|Rat |
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Product Format | Liquid |
Clonality | Polyclonal |
Host | Rabbit |
Application | Enzyme-linked immunosorbent assay|Western blot |
Reconstitution and Storage | -20°C |
Immunogen | The antiserum was produced against synthesized peptide derived from human Ezrin around the phosphorylation site of Thr566. |
Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
Specificity | Ezrin (phospho-Tyr566) antibody detects endogenous levels of Ezrin only when phosphorylated at tyrosine 566. |
Formulation | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Dilution | The optimal working dilution should be determined by the end user. |
Application Info | WB: 1:500~1:3000; ELISA: 1:20000 |
Reference | Zhou R, et al. (2005) J Cell Sci: 118 (Pt 19): 4381-91 Shiue H, et al.(2005) J Biol Chem: 280(2): 1688-95 Zhao H, et al. (2004) Proc Natl Acad Sci U S A: 101(25): 9485-90 Fievet BT, et al. (2004) J Cell Biol: 164(5): 653-9 |
Gene Symbol | EZR |
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Gene Full Name | ezrin |
Alias Symbols | CVIL;CVL;Cytovillin;cytovillin 2;epididymis secretory protein Li 105;ezrin;HEL-S-105;p81;VIL2;villin 2 (ezrin);Villin-2. |
NCBI Gene Id | 7430 |
Protein Name | Ezrin |
Description of Target | Probably involved in connections of major cytoskeletal structures to the plasma membrane. In epithelial cells, required for the formation of microvilli and membrane ruffles on the apical pole. Along with PLEKHG6, required for normal macropinocytosis. |
Uniprot ID | P15311 |
Protein Accession # | NP_001104547.1 |
Molecular Weight | 69 kDa |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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See our General FAQ page.
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What is the species homology for "EZR Antibody (Phospho-Tyr566) (OAAF00316)"?
The tested species reactivity for this item is "". This antibody is predicted to have homology to "Human|Mouse|Rat".
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How long will it take to receive "EZR Antibody (Phospho-Tyr566) (OAAF00316)"?
This item is available "Domestic: within 1-2 week delivery | International: 1-2 weeks".
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What buffer format is "EZR Antibody (Phospho-Tyr566) (OAAF00316)" provided in?
This item is provided in "Liquid".
Additional format options may be available. For more information please contact info@avivasysbio.com. -
What are other names for "EZR Antibody (Phospho-Tyr566) (OAAF00316)"?
This target may also be called "CVIL;CVL;Cytovillin;cytovillin 2;epididymis secretory protein Li 105;ezrin;HEL-S-105;p81;VIL2;villin 2 (ezrin);Villin-2." in publications.
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What is the shipping cost for "EZR Antibody (Phospho-Tyr566) (OAAF00316)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
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What is the guarantee for "EZR Antibody (Phospho-Tyr566) (OAAF00316)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
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Can I get bulk pricing for "EZR Antibody (Phospho-Tyr566) (OAAF00316)"?
You can get bulk pricing for this item by going here.
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What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "69 kDa".
Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics. -
What protocols are available for "EZR Antibody (Phospho-Tyr566) (OAAF00316)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
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What are positive controls for "EZR"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What are negative controls for "EZR"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What other proteins interact with "EZR"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
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What biological processes are associated with "EZR"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
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What cellular components are associated with "EZR"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
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What protein functions are associated with "EZR"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.