EZH2 Antibody (OADC00049)

Catalog No: OADC00049

Size:50ug

ChIP results obtained with the Aviva monoclonal antibody directed against EZH2. ChIP assays were performed using 2.5 ug of the Aviva monoclonal antibody against EZH2 on sheared chromatin from 5 million E14 mouse embryonic stem and from SUZ12-/- cells, used as a negative control. IgG was used as negative IP control. Quantitative PCR was performed with primers specific for the HoxA10 gene and for the T gene which encodes the Brachyury transcription factor. Figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).

Product Info

• Predicted Species Reactivity: Human
• Product Format: Liquid. Provided in PBS containing 0.05% azide.
• Clonality: Monoclonal
• Isotype: Isotype: IgG1*
• Host: Mouse
• Conjugation: Unconjugated
• Application: CHIP
• Reconstitution and Storage: Store at -20C. Avoid repeated freeze/thaw cycles.
• Purification: Protein G purified
• Concentration: 1 mg/mL
• Preservative: 0.05% Sodium Azide
• Specificity: Mouse monoclonal antibody raised against NR3C1
• Application Info: CHIP: 2.5 ug/CHIP

Target Info

• Gene Symbol: EZH2
• Gene Full Name: enhancer of zeste 2 polycomb repressive complex 2 subunit
• Alias Symbols: WVS, ENX1, KMT6, WVS2, ENX-1, EZH2b, KMT6A
• NCBI Gene Id: 2146;
• Protein Name: histone-lysine N-methyltransferase EZH2
• Description of Target: This gene encodes a member of the Polycomb-group (PcG) family. PcG family members form multimeric protein complexes, which are involved in maintaining the transcriptional repressive state of genes over successive cell generations. This protein associates with the embryonic ectoderm development protein, the VAV1 oncoprotein, and the X-linked nuclear protein. This protein may play a role in the hematopoietic and central nervous systems. Multiple alternatively splcied transcript variants encoding distinct isoforms have been identified for this gene.
• Uniprot ID: Q15910
• Protein Accession #: NP_001190176.1
• Nucleotide Accession #: NM_001203247.1