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Catalog No: OKEH04187
Size:96T
Price: $525.00
SKU
OKEH04187
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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  • $55: Antibody & Protein in US
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHomo sapiens, Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology ESRRG ELISA Kit (Human) (OKEH04187) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for ESRRG has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for ESRRG is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample ESRRG captured in the well.
ELISA Kit Range0.312-20ng/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 94%
ELISA Kit ReproducibilityMean Intra-assay CV%: <= 5.3% (n = 20)
Mean Inter-assay CV%: <= 9.5% (n = 20)
ELISA Kit Component
ComponentAmount
ESRRG Microplate96 Wells (12 x 8 Well Strips)
ESRRG Standard2 x 2 ng
Sample Diluent1 x 20 mL
ESRRG Biotinylated Detector Antibody1 x 120 uL
Detector Antibody Diluent1 x 12 mL
HRP Avidin Conjugate1 x 120 uL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue homogenates, Cell culture supernates and Other biological fluids.
Sensitivity0.12 ng/mL
SpecificityNatural and recombinant Human Estrogen-related receptor gamma
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolESRRG
Gene Full Nameestrogen related receptor gamma
Alias SymbolsERR gamma-2;ERR3;ERRg;ERRgamma;ERR-gamma;estrogen receptor-related protein 3;estrogen-related receptor gamma;NR3B3;nuclear receptor subfamily 3 group B member 3.
NCBI Gene Id2104
Protein NameP62508
Description of TargetEstrogen-related receptor gamma
Uniprot IDhttps://www.uniprot.org/uniprot/P62508
Protein Accession #NP_001127757.1
Nucleotide Accession #NM_001134285.2
Protein Size (# AA)458
Molecular Weight51 kDa
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