ESR1 ELISA Kit (Chicken) (OKEH04619)

Catalog No: OKEH04619

Size:96T

Chicken ESR1 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Chicken, Gallus gallus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~3 Hours
• ELISA Kit Principle: Aviva Systems Biology ESR1 ELISA Kit (Chicken) (OKEH04619) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for ESR1 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for ESR1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample ESR1 captured in well.
• ELISA Kit Range: 0.312-20ng/mL
• ELISA Kit Reproducibility: Mean Intra-Assay CV: < 10%
  Mean Inter-Assay CV: < 12%
• ELISA Kit Component:
  • Anti-ESR1 Microplate: 96 Wells (12 x 8 Well Strips)
  • ESR1 Lyophilized Standard: 2
  • Sample Diluent: 1 x 20 mL
  • ESR1 Biotinylated Detector Antibody: 1 x 60 uL
  • Detector Antibody Diluent: 1 x 12 mL
  • HRP Avidin Conjugate: 1 x 120 uL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: serum, Plasma, Urine, tissue homogenates and Cell culture supernates and Other biological fluids
• Sensitivity: 0.084 ng/mL
• Specificity: Natural and recombinant Chicken Estrogen receptor
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: ESR1
• Gene Full Name: estrogen receptor 1
• Alias Symbols: ER;ER-alpha;estradiol receptor;estrogen receptor;estrogen receptor, alpha;nuclear receptor subfamily 3 group A member 1.
• NCBI Gene Id: 396099
• Protein Name: P06212
• Description of Target: Estrogen receptor
• Uniprot ID: https://www.uniprot.org/uniprot/P06212
• Protein Accession #: NP_990514.1
• Nucleotide Accession #: NM_205183.2
• Protein Size (# AA): 589
• Molecular Weight: 67 KDa