ESAM ELISA Kit (Human) (OKCD00940)

Catalog No: OKCD00940 (Formerly GWB-KBBTY4)

Size:96WELLS

Human ESAM ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Endothelial Cell Adhesion Molecule (ESAM). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Endothelial Cell Adhesion Molecule (ESAM). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Endothelial Cell Adhesion Molecule (ESAM), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Endothelial Cell Adhesion Molecule (ESAM) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.312-20ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Endothelial Cell Adhesion Molecule (ESAM) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Endothelial Cell Adhesion Molecule (ESAM) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-ESAM Microplate: 96 Wells (12 x 8 Well Strips)
  • ESAM Lyophilized Standard: 2 x 20 ng
  • 100X Biotinylated ESAM Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Tissue homogenates, cell lysates and other biological fluids
• Sensitivity: < 0.114 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Endothelial Cell Adhesion Molecule (ESAM). No significant cross-reactivity or interference between Endothelial Cell Adhesion Molecule (ESAM) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: ESAM
• Gene Full Name: endothelial cell adhesion molecule
• Alias Symbols: 2310008D05Rik;endothelial cell-selective adhesion molecule;HUEL (C4orf1)-interacting protein;LP4791 protein;W117m.
• NCBI Gene Id: 90952
• Protein Name: Endothelial cell-selective adhesion molecule
• Description of Target: Can mediate aggregation most likely through a homophilic molecular interaction.
• Uniprot ID: Q96AP7
• Protein Accession #: NP_620411
• Nucleotide Accession #: NM_138961.2
• Protein Size (# AA): 390