ERLIN2 ELISA Kit (Human) (OKCD01068)

Catalog No: OKCD01068 (Formerly GWB-KBBHV6)

Size:96WELLS

Human ERLIN2 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Endoplasmic Reticulum Lipid Raft Associated Protein 2 (ERLIN2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Endoplasmic Reticulum Lipid Raft Associated Protein 2 (ERLIN2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Endoplasmic Reticulum Lipid Raft Associated Protein 2 (ERLIN2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Endoplasmic Reticulum Lipid Raft Associated Protein 2 (ERLIN2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.312-20ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Endoplasmic Reticulum Lipid Raft Associated Protein 2 (ERLIN2) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Endoplasmic Reticulum Lipid Raft Associated Protein 2 (ERLIN2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-ERLIN2 Microplate: 96 Wells (12 x 8 Well Strips)
  • ERLIN2 Lyophilized Standard: 2 x 20 ng
  • 100X Biotinylated ERLIN2 Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Tissue homogenates and other biological fluids.
• Sensitivity: < 0.115 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Endoplasmic Reticulum Lipid Raft Associated Protein 2 (ERLIN2). No significant cross-reactivity or interference between Endoplasmic Reticulum Lipid Raft Associated Protein 2 (ERLIN2) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: ERLIN2
• Gene Full Name: ER lipid raft associated 2
• Alias Symbols: C8orf2;endoplasmic reticulum lipid raft-associated protein 2;epididymis secretory sperm binding protein;erlin-2;NET32;spastic paraplegia 18 (autosomal dominant);SPFH domain family, member 2;SPFH2;SPG18;stomatin-prohibitin-flotillin-HflC/K domain-containing protein 2.
• NCBI Gene Id: 11160
• Protein Name: Erlin-2
• Description of Target: Component of the ERLIN1/ERLIN2 complex which mediates the endoplasmic reticulum-associated degradation (ERAD) of inositol 1,4,5-trisphosphate receptors (IP3Rs) such as ITPR1 (PubMed:19240031, PubMed:17502376). Promotes sterol-accelerated ERAD of HMGCR probably implicating an AMFR/gp78-containing ubiquitin ligase complex (PubMed:21343306). Involved in regulation of cellular cholesterol homeostasis by regulation the SREBP signaling pathway. May promote ER retention of the SCAP-SREBF complex (PubMed:24217618).
• Uniprot ID: O94905
• Protein Accession #: NP_001003790
• Nucleotide Accession #: NM_001003790.3
• Protein Size (# AA): 339