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Catalog No: OKEH04740
Size:96T
Price: $525.00
SKU
OKEH04740
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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  • $55: Antibody & Protein in US
  • $55 + $25/Kit in US
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHomo sapiens, Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~3 Hours
ELISA Kit LinearityMean recovery when spiking into serum: 114%
ELISA Kit PrincipleAviva Systems Biology EREG ELISA Kit (Human) (OKEH04740) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for EREG has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for EREG is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample EREG captured in well.
ELISA Kit Range15.6-1000pg/mL
ELISA Kit ReproducibilityMean Intra-Assay CV: < 6.0% (n=20)
Mean Inter-Assay CV: < 10.5% (n=24)
ELISA Kit Component
ComponentAmount
EREG Microplate96 Wells (12 x 8 Well Strips)
EREG Standard2
Sample Diluent1 x 20 mL
EREG Biotinylated Detector Antibody1 x 120 uL
Detector Antibody Diluent1 x 12 mL
HRP Avidin Conjugate1 x 120 uL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue homogenates, Cell culture supernates and Other biological fluids.
Sensitivity10 pg/mL
SpecificityNatural and recombinant Human Proepiregulin
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolEREG
Gene Full Nameepiregulin
Alias SymbolsEp;EPR;ER;proepiregulin.
NCBI Gene Id2069
Protein NameO14944
Description of TargetProepiregulin
Uniprot IDhttps://www.uniprot.org/uniprot/O14944
Protein Accession #NP_001423.1
Nucleotide Accession #NM_001432.2
Protein Size (# AA)169
Molecular Weight19 kDa
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