ERCC8 Antibody (OAGA00016)

Catalog No: OAGA00016

Size:100 ul

Sample(30 ?g of whole cell lysate)
A:293T
B:A431
12% SDS PAGE
OAGA00016 diluted at 1:500

ERCC8 antibody [N2C2], Internal detects ERCC8 protein by Western blot analysis.
A. 30 ug Neuro2A whole cell lysate/extract
B. 30 ug GL261 whole cell lysate/extract
C. 30 ug C8D30 whole cell lysate/extract
D. 30 ug NIH-3T3 whole cell lysate/extract
E. 30 ug Raw264.7 whole cell lysate/extract
F. 30 ug C2C12 whole cell lysate/extract
10 % SDS-PAGE
ERCC8 antibody [N2C2], Internal (OAGA00016) dilution: 1:1000

Immunofluorescence analysis of paraformaldehyde-fixed A431, using ERCC8(CSA)(OAGA00016) antibody at 1:200 dilution.

Product Info

• Predicted Species Reactivity: Human, Mouse
• Product Format: Liquid
• Clonality: Polyclonal
• Isotype: IgG
• Host: Rabbit
• Application: ICC, IF, WB
• Additional Information: Background: This gene encodes a WD repeat protein, which interacts with Cockayne syndrome type B (CSB) protein and with p44 protein, a subunit of the RNA polymerase II transcription factor IIH. Mutations in this gene have been identified in patients with hereditary disease Cockayne syndrome (CS). CS cells are abnormally sensitive to ultraviolet radiation and are defective in the repair of transcriptionally active genes. [provided by RefSeq]
  Positive Controls: 293T, A431, Neuro2A, GL261, C8D30, NIH-3T3, Raw264.7, C2C12
  Cellular Localization: Nucleus
• Reconstitution and Storage: Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
• Immunogen: Recombinant fragment corresponding to a region within amino acids 106 and 330 of CSA (Uniprot ID#Q13216)
• Purification: Purified by antigen-affinity chromatography.
• Concentration: 1 mg/ml
• Application Info: Suggested starting dilutions are as follows: WB: 1:500-1:3000, ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
• Storage Buffer: 1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Target Info

• Gene Symbol: ERCC8
• Gene Full Name: DNA excision repair protein ERCC-8
• Alias Symbols: CSA, CKN1, UVSS2
• NCBI Gene Id: 1161
• Protein Name: DNA excision repair protein ERCC-8
• Uniprot ID: Q13216
• Protein Accession #: NP_000073.1
• Molecular Weight: 44 kDa