Catalog No: OKCD00299 (Formerly GWB-KBBZF5)
Size:96WELLS
Price: $675.00
SKU
OKCD00299
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Datasheets/ManualsPrintable datasheet for ERCC2 ELISA Kit (Human) (OKCD00299)
Product Info
Predicted Species ReactivityHomo sapiens|Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit Linearity
Sample1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
86-98%94-105%94-103%94-102%
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Xeroderma Pigmentosum, Complementation Group D (XPD). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Xeroderma Pigmentosum, Complementation Group D (XPD). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Xeroderma Pigmentosum, Complementation Group D (XPD), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Xeroderma Pigmentosum, Complementation Group D (XPD) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.156-10ng/mL
ELISA Kit Recovery
MatrixRecovery Range (%)Average (%)
83-9390
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Xeroderma Pigmentosum, Complementation Group D (XPD) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Xeroderma Pigmentosum, Complementation Group D (XPD) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-ERCC2 Microplate96 Wells (12 x 8 Well Strips)
ERCC2 Lyophilized Standard2 x 10 ng
100X Biotinylated ERCC2 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeTissue homogenates, cell lysates and other biological fluids.
Sensitivity< 0.057 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Xeroderma Pigmentosum, Complementation Group D (XPD). No significant cross-reactivity or interference between Xeroderma Pigmentosum, Complementation Group D (XPD) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
Gene SymbolERCC2
Gene Full NameERCC excision repair 2, TFIIH core complex helicase subunit
Alias Symbolsbasic transcription factor 2 80 kDa subunit;BTF2 p80;COFS2;CXPD;DNA excision repair protein ERCC-2;DNA repair protein complementing XP-D cells;EM9;excision repair cross-complementation group 2;excision repair cross-complementing rodent repair deficiency, complementation group 2;general transcription and DNA repair factor IIH helicase subunit XPD;TFIIH;TFIIH 80 kDa subunit;TFIIH basal transcription factor complex 80 kDa subunit;TFIIH basal transcription factor complex helicase subunit;TFIIH basal transcription factor complex helicase XPB subunit;TFIIH basal transcription factor complex helicase XPD subunit;TFIIH p80;TFIIH subunit XPD;TTD;TTD1;xeroderma pigmentosum complementary group D;xeroderma pigmentosum group D-complementing protein;XPD.
NCBI Gene Id2068
Protein NameGeneral transcription and DNA repair factor IIH helicase subunit XPD
Description of TargetATP-dependent 5'-3' DNA helicase, component of the general transcription and DNA repair factor IIH (TFIIH) core complex, which is involved in general and transcription-coupled nucleotide excision repair (NER) of damaged DNA and, when complexed to CAK, in RNA transcription by RNA polymerase II. In NER, TFIIH acts by opening DNA around the lesion to allow the excision of the damaged oligonucleotide and its replacement by a new DNA fragment. The ATP-dependent helicase activity of XPD/ERCC2 is required for DNA opening. In transcription, TFIIH has an essential role in transcription initiation. When the pre-initiation complex (PIC) has been established, TFIIH is required for promoter opening and promoter escape. Phosphorylation of the C-terminal tail (CTD) of the largest subunit of RNA polymerase II by the kinase module CAK controls the initiation of transcription. XPD/ERCC2 acts by forming a bridge between CAK and the core-TFIIH complex. Involved in the regulation of vitamin-D receptor activity. As part of the mitotic spindle-associated MMXD complex it plays a role in chromosome segregation. Might have a role in aging process and could play a causative role in the generation of skin cancers.
Uniprot IDP18074
Protein Accession #NP_000391
Nucleotide Accession #NM_000400.3
Protein Size (# AA)760
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