ERBB2 Antibody (OAPB00881)

Catalog No: OAPB00881

Size:100UG

Western blot analysis of erbB-2 in rat liver tissue lysate with erbB-2 antibody at (A) 1 and (B) 2 ug/mL.

Immunohistochemistry of erbB-2 in human liver tissue with erbB-2 antibody at 2.5 ug/mL.

Product Info

• Predicted Species Reactivity: Human|Mouse|Rat
• Product Format: Liquid. erbB-2 Antibody is supplied in PBS containing 0.02% sodium azide.
• Clonality: Polyclonal
• Isotype: IgY
• Host: chicken
• Conjugation: Unconjugated
• Application: Enzyme-linked immunosorbent assay|Immunofluorescence|Immunohistochemistry-Paraffin|Immunoprecipitation|Western blot
  Positive Controls: Rat Liver Tissue Lysate
• Reconstitution and Storage: erbB-2 antibody can be stored at 4C for three months and -20C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Immunogen: erbB-2 antibody was raised against a 19 amino acid synthetic peptide near the carboxy terminus of human erbB-2. The immunogen is located within the last 50 amino acids of erbB-2.
• Purification: Affinity Purified
• Concentration: 1 mg/ml
• Blocking Peptide: For anti-ERBB2 (OAPB00881) antibody is Catalog # OEPB03003
• Application Info: WB: 1-2 ug/mL
  IHC-P: 2.5 ug/mL
  IF: 20 ug/mL
• Reference: Baselga J and Swain SM. Novel anticancer targets: revisiting ERBB2 and discovering ERBB3. Nature Rev.2009; 9:463-475.;Slamon DJ, Clark GM, Wong SG, et al. Human breast cancer: correlation of relapse and survival with amplification of the HER-2/neu oncogene. Science1987; 235:177-82.;Vermeij J, Teugels E, Bourgain C, et al. Genomic activation of the EGFR and HER2-neu genes in a significant proportion of invasive epithelial ovarian cancers. BMC Cancer2008; 8:3.;Marmor MD, Skaria KB, and Yarden Y. Signal transduction and oncogenesis by ErbB/HER receptors. Int. J. Rad. Oncol. Biol. Phys.2004; 58:903-13.

Target Info

• Gene Symbol: ERBB2
• Gene Full Name: erb-b2 receptor tyrosine kinase 2
• Alias Symbols: CD340;c-erb B2/neu protein;HER2;HER-2;HER-2/neu;herstatin;human epidermal growth factor receptor 2;metastatic lymph node gene 19 protein;MLN 19;NEU;neuro/glioblastoma derived oncogene homolog;neuroblastoma/glioblastoma derived oncogene homolog;NGL;p185erbB2;proto-oncogene c-ErbB-2;proto-oncogene Neu;receptor tyrosine-protein kinase erbB-2;TKR1;tyrosine kinase-type cell surface receptor HER2;v-erb-b2 avian erythroblastic leukemia viral oncogene homolog 2;v-erb-b2 avian erythroblastic leukemia viral oncoprotein 2;v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog;VSCN2.
• NCBI Gene Id: 2064
• Protein Name: Receptor tyrosine-protein kinase erbB-2|receptor tyrosine-protein kinase erbB-2 isoform a precursor [Homo sapiens]
• Description of Target: Protein tyrosine kinase that is part of several cell surface receptor complexes, but that apparently needs a coreceptor for ligand binding. Essential component of a neuregulin-receptor complex, although neuregulins do not interact with it alone. GP30 is a potential ligand for this receptor. Regulates outgrowth and stabilization of peripheral microtubules (MTs). Upon ERBB2 activation, the MEMO1-RHOA-DIAPH1 signaling pathway elicits the phosphorylation and thus the inhibition of GSK3B at cell membrane. This prevents the phosphorylation of APC and CLASP2, allowing its association with the cell membrane. In turn, membrane-bound APC allows the localization of MACF1 to the cell membrane, which is required for microtubule capture and stabilization.
• Uniprot ID: P04626
• Protein Accession #: https://www.ncbi.nlm.nih.gov/protein/NP_004439
• Nucleotide Accession #: NM_001005862.2; NM_001289936.1; NM_001289938.1; NM_004448.3